The Quantifiler Trio Quantification Kit has been developed to quantify the total amount of amplifiable and human male DNA in samples and to estimate the extent of DNA degradation. To minimize the cost of DNA quantification, we evaluated kit performance using a reduced volume of reagents (1/10-volume) using DNA samples of varying types and concentrations. Our results demonstrated concordance between the manufacturer's method and the low-volume method for DNA quantification, DNA degradation index estimation, and human male DNA quantification. We confirmed the practical utility of the low-volume method with 109 casework samples by evaluating short tandem repeat (STR) profiling success with respect to DNA quantity and quality. We also defined a cutoff value for DNA quantity to ensure reliable STR results. Using a reduced volume of reagents, 10 times more reactions per kit are possible; accordingly, this method reduces the cost of DNA quantification, while maintaining performance.

Short Tandem Repeats (STR) analysis which characterized by genetic polymorphism has been widely used in the forensic genetic fields. Unfortunately, mutation occurred in various STR loci could make it difficult to interpret STR data. Thus, the mutation rate of STR loci plays an important role for the data interpretation in human identification and paternity test. To verify the mutation of the STR loci in the Korean population, 545 trio sets (father, mother, and child) were analyzed with two commercial STR kits that include the 23 autosomal STR loci (D1S1656, TPOX, D2S441,

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This study establishes the initial data to develop a well-fitted underwear pattern by categorizing and analyzing torso types based on body measurements of women in their 50s and 60s. The results are as follows: First, the statistical assessment on the body measurements showed meaningful differences among age groups in twenty seven items (except for bust breadth, hip width armscye depth, hip depth, neck base circumference, armscye circumference, chest circumference, hip circumference, bishoulder length, shoulder length, front interscye, back interscye, weight and inclined angle of left shoulder). Women in their early 50s and late 60s (respectively) showed the highest values in height and depth. Second, there are five body factors according to the results of the factor analysis: Factor 1 (circumference, width, and depth of upper body measurements) -the degree of body depth and obesity, Factor 2 (height and vertical length) -The vertical torso length, Factor 3 -the size of shoulder, Factor 4 -the vertical upper body length, and Factor 5 -the size of shoulder angle. Third, the results of the cluster analysis showed that there are four distinctive body types. The largest number of the study subjects was related to Type 3 (30.69%), followed by Type 2 (26.78%), Type 1 (25.84%), and Type 4 (16.69%), respectively. For distribution of age groups by body type, Type 3 was the most common among the 60s group while Type 2 appeared most frequently among the 50s.

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