Purpose: Porcine proliferative enteropathy(PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE in a pig farm in earlier stage, a rapid diagnosing test equipment is needed for farmers. To test the equipment appropriately, we prepare the samples and antibodies for rapid detecting the Lawsonia intracellularis in pig feces. Methods : To prepare the PPE infected samples, we sampled PPE suspected pig feces in a pig farm. To manufacture a anti-Lawsonia intracellularis antibody for capturing the Lawsonia intracellularis, the rabbit-anti LsaA synthetic peptide polyclonal antibody was inoculated to rabbits. To select the couple of antibodies which is most well sandwiched with the bacteria, ELISA test was done with PPE infected ileum samples. Finally, to verify the PPE infected feces which would be used to test the rapid kit, PCR test was done on the sampled PPE suspected feces Results : The rabbit-anti LsaA synthetic peptide polyclonal antibody is developed, and is verified to capture the bacterial well through the fluorescence antibody test. Also, we found that the monoclonal antibody and the polyclonal antibody could be used as couples for sandwiching the bacteria. Finally, through the PCR test for samples of pig feces, we could prepare the 150 PPE positive samples and 50 PPE negative samples. Conclusions : The manufactured polyclonal antibody and the imported monoclonal antibody could be used to capture the bacteria using the sandwich techniques. Also, the prepared PPE infected negative and positive samples could be used to test the performance of the rapid kit to capture the bacterium Lawsonia intracellularis Keywords: Porcine proliferative enteropathy (PPE), rapid diagnosis, pig feces, Lawsonia intracellularis Original ArticleJournal of Biosystems Engineering

Purpose: In EU, the animal welfare regulations are strengthening. Also, in Korea, for strengthening of animal protection laws, animal welfare farm certification scheme is being prepared to enforce. To get the certification of animal welfare farm, especially for sows, individual specific feeding in sow group is necessary in accordance with the animal welfare regulation. Also, liquid feeding is reported to be more effective for farrowing as well as dry sow. Therefore, in this study, a electronic liquid sow feeder using RFID which could be fed to farrowing sow simultaneously is developed. Methods : We manufactured a prototype of appropriate elements which were evaluated in the preliminary test for selecting RFID tag, supply pump, mixing type, etc. With the prototype, the performance test for liquid feeding gilt was done in group raising training barn. Results :The performance test result shows the C.V.(coefficient of variation) of liquid mixing and feeding is 0.77~1.97% and 2.4~5.3%, respectively, which means the system could feed sows uniformly. The feeding time of pneumatically activated ball valve is 2 seconds per 0.9 kg of liquid feed. The eating time for gilt were 65 seconds in average. Conclusions : The prototype could feed sow uniformly, and deliver the liquid feed 9 times for a sow continuously. Also, total eating time for a sow was 18 minutes in one visit to the prototype of feeding station. Keywords: Animal welfare, Sow feeding, RFID, Liquid feeding Original ArticleJournal of Biosystems Engineering