No abstract

Coronary heart disease poses one of the most serious threats to human health resulting in enormous physical and economic losses worldwide. WNT signaling pathways play an important role in cardiogenesis both in embryogenesis and cardiac repair after previous ischemic attacks that motivated to conduct this study. The aim of the study was to examine features of WIF-1 production in patients with coronary heart disease. There were enrolled 60 patients with a clinically verified and diagnosed coronary artery disease. WIF-1 serum concentration was measured by using enzyme-linked immunosorbent assay presenting data as absolute numbers (n, %) or medians, 1 and 3 quartiles – Me (Q0.25-Q0.75). Analyzing study data showed that WIF-1 serum concentration in patients with myocardial infarction was 2890 (1700-3337.5) pg/ml being by 7.97-fold higher than that one in healthy individuals (p 0.001), in agreement with previous studies. Moreover, in patients with angina pectoris WIF-1 serum level comprised 2170 (1493-2650) pg/ml, exceeding that one in healthy individuals by 6.14-fold (p 0.001). Thus, the data obtained regarding changes in serum WNT-inhibiting factor-1 concentration in patients with coronary heart disease expand our understanding about an impact from affected WNT-signaling pathway components in pathogenesis of inflammatory process during hypoxic injuries.

The paper presents the results of research on the development of zoohygienic measures aimed at improving the hatchability of eggs through the use of environmentally safe means and the study of the toxic effects of formaldehyde on the quality of hatching eggs. The research is relevant due to the negative changes in industrial poultry farming in recent years, which have been accompanied by a sharp deterioration in the quality of hatching eggs, a decrease in the hatching of conditioned chicks and their significant mortality during the rearing period. The use of the drug "Monclavit-1" as an antiseptic and disinfectant drug of broad spectrum and the study of formaldehyde toxicity in the treatment of hatching eggs is of scientific interest to improve the incubation quality of eggs. The work gives a comparative assessment of the use of formaldehyde for disinfection of hatching eggs and environmentally safe preparation "Monclavit-1". The data obtained and their analysis indicate that preincubation treatment with Monclavit-1 had a positive effect on the results of incubation. Eggs with blood ring in the control group were 0.9% more (p < 0.05), dead-in-shell eggs were 1.3% more (p < 0.05), and addle eggs were 2.0% more (p < 0.05) than in the experimental group. In addition, the rates of egg hatchability and hatchability of chicks when treated with Monclavit-1 were higher than those of the control by 3.1 and 4.4% (p< 0.05), respectively. Cost-effectiveness of the drug "Monclavit-1" use exceeded that of the control group by 17.3%. Production sanitation in the agro-industrial complex is one of the decisive factors in preserving and increasing the health of farm animals and obtaining biologically and environmentally safe products from them to meet the food needs of the population of the state.

The indicators of productivity, survival rate and behavior of young bulls of Hereford breed were studied in a comparative aspect when they were raised in winter in the northern part of Kolpashevsky district of the Tomsk region. During the housing period, low average air temperatures were observed: from 20°С in October to –50°С in December – January and –49°С in February. Bulls of the control group were kept in a unit of the "three-wall" type during wintering, while bulls of the experimental group – in a lightweight unit. The increase in live weight of bulls of experimental group was 2.2 times higher than that in the control group, the slaughter weight was by 68.3 kg and the weight of internal fat was by 6.0 kg more than in the control. In the experimental group, the survival rate of bulls before slaughtering was 2 times higher than that in the control. In terms of live weight, experimental animals from the age of 15 months to slaughter exceeded the control group by 19%. Ethological studies showed that different types of housing units had a significant impact on animal behavior. The feed intake of the bulls of the control group was shorter by 54 minutes, (15%) than that of the experimental group. Animals also spent 95 minutes (20.21%) less on chewing cud compared to the experimental bulls. When kept in a lightweight type of unit, bulls were in motion for a longer time, as a result of which they rested 45 minutes less, which contributed to the increase in the metabolic processes in the body. The animals of the experimental group spent 50 minutes longer on eating food in comparison with the bulls in the “three-wall” unit. The air temperature in a unit of the "three-wall" type was close to the ambient air temperature, wherein the bulls spent most of their time lying down. The profitability of growing bulls in the experimental group exceeded the control indicator by 1.6 times. In the light-type premises, the housing conditions of bulls in the winter period at extremely low air temperatures were most favorable.