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Comparison of activity of antibiotics against Staphylococcus aureus strains in their plankton form and in biofilms of varying maturity showed that the concentrations of antibiotics causing death of 90% S. aureus strains (MIC 90 ) in their plankton form was 0.07-2.80 µg/ml and significantly (p<0.05) differed from MIC 90 concentrations (3-245 µg/ml) for the strains in 24 and 48 h biofilms. MIC 90 value was affected by the degree of biofilm maturity: microbial resistance in 48-h biofilms for all analyzed antibiotics was significantly (p<0.05) higher than in 24-h biofilms.

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