The objective of the study was to determine the needs analysis of biodiversity encyclopedia on Mount Ungaran Central Java. This research method was descriptive qualitative. This research method using questionnaires and interviews to obtain data with a Likert Scale. The sampling technique is purposive sampling by considering the biological abilities of students. The total population of all students of class X MIPA SMAN 2 Ungaran is 210. The sample is 58 people from each of the 6 classes and class X biology teachers. The results of the analysis showed that the students are currently using the learning resources in the form of textbooks, Student worksheets (LKS), and PowerPoint (PPT). The use of books is still less clearly describing biodiversity based on the potential of the school’s environment. as much as 63,79% reveal that the material biodiversity yet has the uniqueness as well as utilizing local potential. As much as 70,68% of students said they need to learn the material biodiversity based on the local potential, especially in Mount Ungaran in Encyclopedia. Therefore, it is necessary to develop learning resources on biodiversity to utilize local schools’ potential.