Objective: The aim was to develop a simple, rapid, less expensive, linear, precise, and accurate reverse phase high performance liquid chromatography method for determination of nifedipine in tablets. Methods:The chromatographic analysis of nifedipine was performed using liquid chromatograph Agilent 1290 Infinity II LC System. Selected conditions were isocratic elution with binary mobile phase consisting of solution methanol and 0.1% trifluoroacetic acid (55:45). Detection was carried out using spectrophotometric detector at 265 nm. The method was validated as per ICH guidelines. Results:The retention time for nifedipine by proposed high performance liquid chromatography (HPLC) method is observed as 3.5 minutes. The correlation coefficients are 1.0000. The developed chromatographic method was found to be accurate with recovery 99.2-99.8% and was found within the acceptance criteria (i.e. 98.0-102.0%) with acceptable % relative standard deviation of not more than 2% at each level. The assay results of nifedipine in tablets by developed method are highly reproducible, reliable and are in good agreement with the label claim of the medicines (average 99.62 %).Conclusion: Finally, it should be noted that a new simple, rapid, linear, precise, accurate HPLC method was developed and validated for the determination of nifedipine in medicines in accordance with the ICH guidelines. These results show the method are accurate, precise, sensitive, economic, and rugged. The proposed HPLC method is rapider (retention time is 3.5 minutes). This method can be useful for the routine analysis of nifedipine in pharmaceutical dosage form.

Objective: The present study was designated to analyse the essential oils and tannins as important secondary metabolites of the aerial part of Satureja hortensis. Methods:The chemical composition of S. hortensis herb was investigated using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The essential oil was obtained by hydrodistillation method. Results:The essential oil yield in the aerial part of S. hortensis was 1.61%. GC-MS analysis of the essential oils identified the presence of 29 components. Carvacrol (76.16%), as the main component of essential oils, belongs to the group of aromatic compounds. Eight tannin components identified by HPLC and epigallocatechin (130.91х10 Ternopil, 46001, Ukraine Email: shanayda-mi@ukr.net -2 %) are prevalent among them. Conclusion: S. hortensis was found to possess considerable amountKeywords: Satureja hortensis, Essential Oil, Tannins, HPLC, GC-MS.of phytoconstituents such as essential oils and tannins. The results of this research will help to study pharmacological properties of the investigated plant and to prevent possible adulteration with other plants.

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Background. Lipid metabolism disorders in the organism