The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 µg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for the enumerating amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacteria groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B1 and hydrolysed fumonisin B1 concentration were analysed by liquid chromatograghy – mass spectrometry. There was no significant difference in FB1 concentration between the experimental and the control 2 group (buffer and fumonisin B1) at 0 h incubation, 5.185 ±0.174 µg/ml compared with 6.433 ±0.076 µg/ml. Fumonisin B1 concentration in the experimental group was reduced to 4.080 ±0.065 µg/ml at 24 h and to 2.747 ±0.548 µg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B1 was detected after 24 h incubation (0.012 ±0 µg/ml). At 48 h incubation time, hydrolysed fumonisin B1 concentration was doubled to 0.024 ±0.004 µg/ml. These results indicate that fumonisin B1 can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.

As the most common grain contaminant worldwide, deoxynivalenol is of high importance despite its low toxicity compared to other trichothecene mycotoxins. Data on the effects of deoxynivalenol in rabbits are scarce. Thus, the aim of this study was to investigate the effects of dietary deoxynivalenol fed at a high level (10 mg/kg of feed) on the productive performance, blood indices, immunological variables, histopathological changes, and genotoxicity in rabbits. Forty-eight Pannon White rabbits were exposed to contaminated diets for three weeks. Despite its high concentration, deoxynivalenol did not affect the feed intake, body weight, and body weight gain. Liver and kidney function was not affected, as shown by the clinical chemistry indices. Conversely, in two rabbits the toxin caused mild fibrosis of the liver, without degenerative changes of the hepatocytes. No genotoxicity could be observed either. Gut cytokines and the phagocytic activity of the macrophages did not differ significantly. The percentage of neutrophils was significantly lower, whereas that of eosinophils was significantly higher in the toxin-fed group. Deoxynivalenol did not cause significant changes in gut and villus morphology. In 4 out of the 6 deoxynivalenol-treated animals, the ratio of lymphoblast proliferation and simultaneous apoptosis shifted towards apoptosis in the gut-associated lymphoid tissue. In the central part of the lymphoid follicles of the spleen, lymphocyte depletion and follicular atrophy could be detected. It can be concluded that rabbits are less sensitive to deoxynivalenol, but the findings confirm that this Fusarium toxin is capable of modulating the immune response. Mycotoxin, immune response, histology, blood indicesMycotoxins are secondary metabolites of filamentous fungi occurring worldwide. Despite being the least toxic trichothecene, the ubiquitous occurrence of deoxynivalenol (DON) in grains increases its importance for food and feed safety. The pig is the most sensitive, whereas ruminants are the least sensitive animal species to DON (Pestka and Smolinski 2005;Pestka 2007;Sobrova et al. 2010). Monogastric animals are extremely prone to growth and body weight gain suppression upon DON exposure. Deoxynivalenol possesses immunomodulatory properties as well (Pestka and Smolinski 2005). To the best of our knowledge, only few experiments have been conducted with DON in rabbits (Khera et al. 1986;Hewitt et al. 2012). The aim of this study, therefore, was to investigate the effects of 10 ppm (mg/kg feed) of dietary DON which is twice as much as the highest guidance value

In this study, aflatoxin B (AFB) toxicity toward the earthworm Eisenia fetida (Savigny 1826) was evaluated in contact paper test systems containing distilled water and ethanol or 20 to 400 μg/ml of AFB over 72 h of exposure. The results indicated that AFB could induce significant damage to earthworms (coiling, curling, excessive mucus secretion, clitellum swelling) at greater than 75 μg/ml. Moreover, AFB had harmful effects on E. fetida (degenerative changes such as bulging of the clitella regions) at levels higher than 150 μg/ml. The calculated LD was 168.5 μg/ml. These findings confirm that E. fetida and standardized methods based on this organism (OECD 207 1984) are applicable and useful in mycotoxin related toxicity studies.

In this study the occurrence of hidden fumonisin B1 (FB1) and fumonisin B2 (FB2) was analysed, on two cereal substrates (maize and rice), inoculated with Fusarium verticillioides (MRC 826), in order to determine the ratio of hidden FB1 and FB2. Two parallel methods were applied: an in vitro human digestion sample pre-treatment and the routine extraction procedure, in both cases with subsequent LC-MS analysis. It was found that all samples showed higher concentration of total fumonisin B1 after digestion, as compared to that of free fumonisin analysed only after extraction. The percentage of the hidden form by maize was 18.8 % (±2.4) for FB1 and 36.8 % (±3.8) for FB2, while for rice it was 32.3 % (±11.3) and 58.0 (±6.8), respectively, expressed as the proportion to total fumonisin B1, for the total dataset. Significant differences were found in the FB1 and FB2 concentration measured after the different digestion phases (saliva, gastric and duodenal) in case of both matrixes. The results are useful for human risk assessment, since both humans and animals may be exposed to markedly higher toxin load, as determined merely by conventional analytical methods.

The research was conducted from August until November 2012 in the districts of Moldavia (Botoşani, Suceava, Iaşi, Vaslui, Bacău, Neamţ, Galaţi and Vrancea), in the West and the Central part of the country (Cluj and Covasna districts) and in the South-East of the country (Tulcea and Brăila districts). The choice of the districts was established based on the informations received from Animal Improvement and Breeding Offices in Romania. The purpose of this research was to identify the effective of Sura de stepă cattle breed in Romania and the evaluation of the phenotypic characters in steppe animals, which belong to the variety of Moldavian breed. The results showed that the Sura de stepă breed consists of a minor part, being raised in just two districts of Moldavia, Iaşi and Neamţ, respectively, as a pure breed with an average of 0,03 % (83 cows), at the Research and Development Station for Cattle Breeding (R.D.S.C.B.) Dancu-Iaşi, with a core of preservation of 59 cows (0,19 %) and at the Holding TCE 3 Brazi Society, Neamţ district, with a core of 24 cows (0,06 %), and under half-breed form with an average of 0,33 % (592 cows). In Harghita, Covasna and Cluj districts, 295 cows from the Sura de stepă breed, Hungarian variety, were identified, excepting being the animals belonging to University of Agricultural Sciences and Veterinary Medicine Cluj, which come from R.D.S.C.B. Dancu-Iaşi, which are Romanian Sura de stepă, Moldavian variety. In Tulcea district, 20 cows of half-breed Sura de stepă and 50 cows of pure breed cattle, Moldavian variety, were identified, from which 24 cows at a private landlord in Pardina locality, Tulcea district, and 26 cows belonging to nine owners from different localities (C.A. Rosetti, Pardina, Chilia Veche, Sfântu Gheorghe, Crişan), each owning 2-3 cows. The analysis of the main body indices showed that the Sura de stepă cows from Pardina, Tulcea county, are of small size and weight, having smaller values in all analysed parameters, compared to the Sura de stepă cows from R.D.S.C.B. Dancu-Iaşi from the North-East part of the country, which however fitted the specific limits of the breed, Moldavian variety.