This study aimed to identify anti-bacterial compounds consisted of the ethyl acetate fraction of Aspergillus sp extracted from the root of Rhizophora mucronata. The sample of Aspergillus sp cultivated from mangrove located in West Sumatera province. Each culture isolated was macerated in the ethyl acetate and analyzed using GC-MS instrumentation. The GC-MS spectra indicated fifteen compounds including isoindole derivative, organic acids (3 compounds), organic acid ester (4 compounds), acetate derivative, acetone derivative, alcohol derivatives (3 compounds) and naphthalene derivative. Among the identified compounds,