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Lactobacillus plantarum strain 8014-H2, unlike other lactobacilli studied, does not require mevalonic acid for growth, but growing and resting cells utilize it only for the synthesis of nonsaponifiable lipids. Upon the incubation of washed cells with mevalolactone-2-C14, the label appeared in the lipids but not in CO2. On the other hand, when mevalolac

No abstract

galactosidase (d-galactoside galactohydrolase, EC 3.2.1.23) is inducible in Lactobacillus plantarum by D-galactose or thiomethyl galactoside, and to a much lesser extent by lactose, isopropyl thiomethyl galactoside, and D-fucose. Isopropyl thiomethyl galactoside is a competitive inhibitor of the enzyme with a K1 of 4.2 mM. The Km of the crude enzyme for o-nitrophenyl fl-D-galactoside is 0.87 6 on July 31, 2020 by guest http://jb.asm.org/ Downloaded from 70