AIM: The purpose of this study was to determine the antibacterial activity of Lumbricus rubellus earthworms through inhibitory zone diameter to the growth of the bacterium Phorphyromonas gingivalis as the cause of periodontitis. METHODS: This was an experimental study with randomised posttest-only control group design. The study was conducted at the Microbiology Research Center laboratory at the Faculty of Dentistry, Airlangga University, Indonesia. The study was conducted in vitro, the sample size was calculated using the Federer formula as many as four agar plates containing bacteria Phorphyromonas gingivalis, with each plate given five different treatments: control (ethanol), Lumbricus rubellus earthworm extract (ECT) with concentrations of 50%, 25%, 12.5%, and 6.25% respectively. The data in the form of inhibition zone diameter (measured in millimetres) obtained were tested using One-Way ANOVA. RESULTS: The mean diameter of the inhibitory zone extract of Lumbricus rubellus earthworm on the growth of Phorphyromonas gingivalis bacteria in the treatment group had significant differences (p < 0.05). The mean inhibition zones between controls and the ECT treatment group (ECT 50%, ECT 25%, ECT 12.5%) were statistically different (p < 0.05), in contrast with ECT 6.25% (p > 0.05) which did not show significant difference with the control group (p > 0.05). CONCLUSION: Lumbricus rubellus earthworm extract with a concentration of 50% has the largest diameter of the inhibitory zone on the growth of the Phorphyromonas gingivalis bacteria. The 6.25% earthworm extract showed no antibacterial activity against the growth of Phorphyromonas gingivalis bacteria.

Cemcem atau Spondias pinnata (L.f) Kurz merupakan tanaman yang banyak digunakan oleh masyarakat Bali sebagai obat tradisional secara turun temurun. Beberapa penelitian telah dilakukan dan didapatkan bahwa ekstrak daun Cemcem memiliki aktivitas antibakteri. P. gingivalis merupakan salah satu penyebab periodontitis. Periodontitis merupakan penyakit gigi dan mulut yang banyak dialami oleh masyarakat Indonesia. Tujuan dari penelitian ini adalah untuk mengetahui kandungan senyawa bioaktif dan daya hambat ekstrak etanol daun Cemcem terhadap pertumbuhan P. gingivalis. Untuk mengetahui kandungan senyawa bioaktif dilakukan uji terhadap alkaloid, flavonoid, terpenoid, steroid, dan tannin. Uji daya hambat ekstrak etanol daun Cemcem dilakukan menggunakan metode difusi cakram dengan kontrol positif yaitu chlorhexidine 2%, kontrol negatif yaitu etanol, serta dua konsentrasi ekstrak yaitu 60% dan 80%. Dari hasil penelitian diperoleh bahwa ekstrak etanol daun Cemcem mengandung senyawa alkaloid, flavonoid, steroid, dan tannin. Zona hambat yang terbentuk memilki perbedaan bermakna antara ketiga perlakuan dan masuk dalam kategori kuat.

Background: Plants that are medical and used for generations are aloe vera and betel leaf, which contain natural chemical and have antimicrobial and antiseptic properties.Objective: The purpose of this study was to calculate the growth inhibition zone of Streptococcus mutans by using the interaction of aloe vera extract and betel leaf, with a concentration of 50%, 75%, 100%.Method: This research was carried out at the Microbiology Laboratory of Udayana University using a pure experimental method of extracts of Aloe vera leaves and betel leaves with concentrations of 50%, 75%, 100%, seven repetitions, using Streptococcus mutans ATCC 35668, and isolation media for Mueller Hilton plus 5 % goat blood.Results: The average inhibition zone diameter in the interaction groups of aloe vera extract and betel leaf in each group was statistically significant difference (p 0.05), where the inhibitory concentration with the highest 100% concentration was an average of 82.6 mm with very strong criteria.Conclusion: The interaction of aloe vera leaf extract and betel leaf with a concentration of 100% has the highest inhibitory power of 82.6 mm, with very strong criteria.

Adolescence is an age stage that comes after childhood ends which is characterized by rapid growth with an age range of 10-19 years. Teenage girls are generally more prone to anemia than teenage boys. This community service activity aims to provide education to teenage girls about anemia and laboratory tests such as Hb levels and urine protein as an effort to provide health services to teenage girls at SMPN 1 Ubud, Gianyar. The method of implementing this activity includes counseling about anemia, checking Hb levels, and urine protein examination, as well as pre-test and post-test to determine the level of knowledge of teenage girls about anemia. From the results of the examination of 34 respondents, it was found that 29.4% had anemia and none of the respondents had proteinuria. As many as 94% of respondents have a good level of knowledge about anemia. There needs to be a role from family, school, and government to improve the health status of teenage girls such as giving blood tablets regularly to avoid anemia.

Oral Hygiene is a free conditions of teeth from plaque and calculus. Oral health is influenced by several factors, one of which factor is regularity of brushing teeth. The purpose of this research is to know OHI-Sdescription and brushing teeth behavior of smoker teenagers in Banjar Tengah, Kukuh village, Marga sub districts, Tabanan regency, 2017. This research is a descriptive are using the total population about 35 smoker teenagers. The result of this research is smoker teenagers with OHI-S good criterias four peoples, 19 peoples includes moderate criteria, and bad criteria seven peoples. The average of smoker teenagers OHI-S is 2,45 with a moderate criteria. Smoker teenagers who behave that teeth brushing behavior with a very good criteria one people, four peoples good criteria, the criteria quite seven peoples, and 32 peoples with need guidance criteria. Based on the results of the study it can be concluded smoker teenagers have a highest OHI-S medium criteria, the average OHI-S 2,45 and the most brushing teeth behavior that need guidance criteria. Suggestion can be given that should  do counseling about how to maintain oral health and counseling about danger and smoking consequence in order to create a good health status.