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Biosensors for cholesterol were prepared by electropolymerization of pyrrole in solutions of 0.1 M phosphate buffer containing 10 mM NaClO, and 15 to 55 IU/mL of cholesterol oxidase at a potential of 800 mV versus a calomel reference electrode (3 M KCl). The sensors were tested for their response toward cholesterol in batch. flow-through, and flow-injection systems by detection of the hydrogen peroxide formed in the enzymatic reaction. Flow-injection analysis (FIA) measurements with an injection loop of 50 pL were performed with a high reproducibility in the range of 0 to 250 pM cholesterol with solutions of constant detergent concentration (1% Triton X-100 in 0.1 M phosphate buffer of pH 7.0) and at a flow rate of 0.2 mL/min. At this flow rate, the detection limit was approximately 5 p M cholesterol and the sample throughput was 25 injections per hour. The response of the sensors toward ascorbic and uric acid was investigated.

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