This study aimed to determine the prevalence and risk factors for children with constipation. This research was an analytic cross-sectional study. The sampling method was cluster sampling. The research was conducted at some kindergartens in Denpasar, Bali from October to November 2019. Data were obtained based on questionnaires distributed to parents and anthropometric examinations. Constipation was defined according to The Rome IV criteria. There were 215 children included in the study and 43 (20%) children had constipation. Children who started toilet training before the age of 1.5 years or after 2 years had 2,73 times the risk of developing constipation (p = 0.019; 95% CI 1.18-6.3). Children with less fiber consumption in a day had the risk of experiencing constipation 7.25 times (p = 0.001; 95% CI 3.38-15.5). Other risk factors such as sex, physical activity, screen contact, water consumption, nutritional status, birth weight, and mother education did not statistically relate to constipation. Toilet training before the age of 1.5 years or after 2 years and low fiber consumption are risk factors for constipation in preschool children in Denpasar.