Дослідження виконано в рамках НДР «Корекція розладів репродуктивної функції при експериментальній моделі простатиту у щурів за допомогою наночастинок на основі оксидів рідкісноземельних елементів» Досліджені зразки наночастинок було синтезовано в Інституті сцинтиляційних матеріалів НАН України на підставі Договору про наукову співпрацю між ДУ «Інститут проблем ендокринної патології ім. В. Я. Данилевського НАМН України» (м. Харків) та Інститутом сцинтиляційних матеріалів НАН України (м. Харків) від 07.02.2018 р. (відділ наноструктурних матеріалів, керівник докт. фіз.-мат. наук, проф., член-кор. НАН України Малюкін Ю. В.). Установою, що фінансує дослідження, є НАМН України. Автор гарантує повну відповідальність за все, що опубліковано в статті. Автор гарантує відсутність конфлікту інтересів і власної фінансової зацікавленості при виконанні роботи та написанні статті. Рукопис надійшов до редакції 27.11.2019.

The anti-aging effects of the rare-earth metals nanoparticles have been studied on the 18-21-month-old rat males. It was shown that the nanoparticles of Gadolinium Ortovanadate activated by Europium (GdVO 4 : Еu 3+) in a dose of 0.33 mg/kg during chronic using (70 days) have demonstrated the anti-aging effects on the old male's reproductive function manifested by the increasing the sex hormone level and activating the spermatogenesis.

Background. The study was aimed to determine the correlation between the blood concentration of vitamin D and factors that influence the function of the endothelium and the hemodynamic of gonads in women with polycystic ovary syndrome (PCOS). Material and methods. Sixty women aged from 18 to 26 years were examined: 30 women with a diagnosis of PCOS and 30 healthy women. The blood serum concentration of vitamin D, C-reactive protein (CRP), interleukin-6 (IL-6), homocysteine (Hcy), nitrites/nitrates (NOx), and arginine were investigated. Maximum systolic velocity (Vmax), resistance index (RI), and pulsatility index (PI) were measured with Doppler ultrasound. Cholecalciferol was used in a dose of 4000 IU per day for 12 weeks for the correction of deficiency of vitamin D. Results. The patients with PCOS were found to have a lower (Р < 0.001) average vitamin D than healthy women. The reduction of vitamin D concentration was combined with an increase (P < 0.001) in the average concentration of NOx and a decrease in arginine. It was found a positive association between vitamin D and arginine (r = 0.391; P < 0.05), between NOx and CRP (r = 0.432; P < 0.02), IL-6 (r = 0.476; P < 0.01), Vmax (r = 0.383; P < 0.05), RI (r = 0.369; P < 0.05), PI (r = 0.380; P < 0.05) and reverse correlation with arginine (r = –0.375; P < 0.05). It was not found an association between NOx and vitamin D (r = 0.207; P > 0.05), Hcy (r = 0.176; P > 0.05); between vitamin D and Vmax (r = 0.231; P > 0.05), RI (r = 0.201; P > 0.05), PI (r = 0.181; P > 0.05). The therapy of cholecalciferol level was accompanied by an increase (P < 0.001) in concentrations of vitamin D, arginine (P < 0.05) and a decrease in NOx (P < 0.02), IL-6 (P < 0.05), SRP (P < 0.001), Hcy (P < 0.001), Vmax (P < 0.001), RI (P < 0.001), PI (P < 0.01), quantity of antral follicles (P < 0.001), and the growth of follicles (P < 0.001). However, none of the studied indicators reached the indicators in healthy women. Conclusions. The deficiency of vitamin D is associated with increased markers of chronic inflammation, homocysteine, nitrate/nitrites, reduced concentration of arginine that provokes the development of endothelium dysfunction and, therefore, the hemodynamics disorders and folliculogenesis in the ovary.

The management of patients with chronic prostatitis (CP) remains the difficult medical and social problem that makes the elaboration of new prostate protectors actual. The purpose of the work: to ascertain the effectiveness of nanoparticles' of rare-earth elements with different form-factors correction of the pathological seminal's endocrine and spermatogenic function disturbances caused by chronic experimental prostatitis. The modeling of CP in mature rats’ males has been fulfilled using Wartner device by cryo trauma of the prostate gland making. The compositions of nanoparticles (NP) of rare-earth metals (LaVO4 or GdVO4 ) activated by Europium, with NP Ag added, in shape of rods, spheres and grains have been chosen for CP correction. The dose was 0,3 mg/kg of body weight. The drug of comparison was 0,5 % «Prostatilen». The researched substances have been rectally introduced in volume of 0,5 ml from 15th after surgery during next two weeks. The number of leukocytes in blood, condition and masses of the prostate gland and seminal vesicles, the concentration of the general testosterone and spermatogenesis indices have been determined. It has been determined that using of NP composition based on the oxides of rare-earth elements and NP Ag (nanorods) for pathology correction has declined consequences of CP: the general inflammation has been substantially inhibited, mass coefficients of the epididymis and testosterone level have been recovered and spermatogenesis have been improved. For some spermogram indices, the activity of these NP does not concede, but even exceeds the activity of drug of comparison. The using of NP GdVO4:Eu3+ + Ag in the spherical shape has positively influenced the correction of chronic prostatitis, although the effectiveness was less distinct. Thus, the composition of nanoparticles LaVO4:Eu3+ + Ag in the form of rods has a positive effect on the sexual function of rats with chronic prostatitis compared with the effect of nanoparticles GdVO4:Eu3++Ag in the form of spheres or grains.