No abstract

We have undertaken the metrological assessment of the method for the quantitative determination of a number of non-steroidal anti-inflammatory drugs (NSAID) including ibuprofen, ketorolac, diclofenac, indomethacin, naproxen, and ketoprofen with the use of gas chromatography mass spectrometry in the model blood samples in the framework of the screening survey of medicinal and narcotic substances. The method was evaluated in terms of the following characteristics: specificity, linearity, correctness, precision (reproducibility), and intra-laboratory precision. The proposed method can be applied for the quantitative evaluation of indomethacin, ketoprofen, and naproxen content as well as for the preliminary quantitative determination of ibuprofen and diclofenac in the framework of the chemico-toxicological and forensic chemical analysis. The method can be employed for the quantitative determination of ketorolac from such characteristics as «linearity» and «reproducibility».

This paper was designed to describe the application of the method for solid-phase extraction of the medicinal and narcotic substances having different physicochemical composition by gas chromatography with mass-spectrometric detection (GC-MS) for the purpose of their screening in the blood. The solid-phase extraction technique was optimized by means of the Box-Behnken modeling with the evaluation of the influence on the effectiveness of extraction of various factors including pH of the buffer solution, eluent composition, the type and the volume of the solutions used to wash the sorbent.

На примере исследования 224 образцов крови показано, что использование методов твердофазной экстракции и газовой хроматографии с масс-спектрометрическим детектированием в скрининге крови позволяет надежно идентифицировать широкий круг наркотических и лекарственных веществ, имеющих судебно-химическое значение, в том числе «дизайнерские» синтетические наркотики — метилендиоксипировалерон (МДПВ), пирролидиновалерофенон (PVP) и их метаболиты; метаболиты каннабимиметиков N-(1-карбамоил-2-метилпропил)-1-пентил-1H-индазол-3-карбоксамида (AB-PINACA) и/или метилового эфира N-[(1-пентил-1H-индазол-3-ил)карбонил]валина (AMB). Для выявленных веществ приведено распределение по фракциям, рассчитаны значения констант диссоциации (pKa) и коэффициентов распределения в системе октанол — вода (log P).