The kinetics of calcium binding by tissue structures was analyzed in 19 healthy volunteers (13 men and 6 women) from the age group of 33 ± 6.5 years under the conditions of acute hypercalcemia followed by drip intravenous infusion of calcium gluconate for 2.5 h. At the end of each 30 min period, the calcium amount retained by the tissue structures was recorded and the kinetic parameters of calcium binding were determined according to Langmuir and Scatchard. In all volunteers, there was a region of binding isotherm with positive cooperativity (linear regression in Scatchard's coordinates) with the same buffer capacity (β tis ) for calcium in Langmuir's coordinates (0.58 ± 0.24 L kg). Half of the volunteers exhibited cooperativity at [Ca 2+ ] = 1.3-1.5 mmol/L, while others, at [Ca 2+ ] = 1.0-1.3 mmol/L, which corresponded to the differences in the association constant (K a ) and the number of interactive sites (n) with [Ca 2+ ] = 1 mmol/L. Additionally, two segments of the binding isotherm were detected with the successive binding of calcium to one set of non interactive centers with similar kinetic parameters of calcium binding (β tis , K a , n). Four different types of cal cium binding in healthy volunteers were found. The results of this study may serve as the basis for a functional diagnostic test of disorders of the tissue calcium binding properties in different pathological conditions.

In this study AlphacaIcidol (Alpha D3) is used in 80 advanced osteoporosis cases. Mean age was 51.5. There were 71 female and 9 male patient. Bone mineral density is the most valuable parameter for sklatal condition of a parson. 2n the study mean Z scot obtained with DEXA (Dual-Photon Absorhtiomatry) and bona density regard to gr/cm2 is compared before and after treatment.Firstly in osteodansitometric measurements is made in ags matched according tO normalls. After young versus adult bona density is compared.alpha calcidol is a drug t~at is especially recomJnendad to be used in cases with reduced alpha hydroxilase enzyme activity.When this enzyme is decreased calciumrsabsorbtJon from the intestines will be raducad.A long with alfa ])3 therapy hypercalcamia is espectad.To prevent this serum calcium, phosphor and alkallnphosphatasa Values are obtained monthly. 27~erapy is started with a dose of 0.25mcg and gradually increased 0.50 and 0.75 and up to Imcg.After reaching that level the dose is again gradually decrased tO 0.25meg and after remaining at this level for i moath the dosage is increased periodically once more. 2n this study BUD evaluation was done in the standard manner (spine + femur). The results was evaluated by spine AP findings. mean mean z scot BIOlD (gr/cm2) young-adult Before treatment -3.4 0.650 66 After " -2. I 0.825 77 P value p<'0.05 p< 0.01 pC 0.05 Z scots, mean BI4Z), young-adult parsanta!ge parameters are compared =statistically before and after treatment. P values were found to be statistically significant. As a result we conclude that Alpha D3, is an effective drug for advanced ostaoporos~s that increases the hone density.