Periodontal disease is a chronic bacterial infection characterized by persistent inflammation, connective tissue breakdown, and alveolar bone destruction. The current study aimed to compare the connective tissue metabolism indices in rats with comorbidity-free periodontitis and in animals with periodontitis in a setting of hyper-and hypothyroidism. 12-14-week-old inbred white male rats (n=48) were included in the experiment. They were randomly divided into the following groups: control, animals with a model of periodontitis, animals with periodontitis in a setting of hyperthyroidism, animals with periodontitis in a setting of hypothyroidism. Serum levels of free thyroxine, free triiodothyronine, and thyroid-stimulating hormone were assayed using ELISA kits manufactured by Vector Best (Russia) to confirm the hyper- and hypothyroid status. Collagenolytic activity, the content of glycosaminoglycans, free hydroxyproline, and fucose, unbound with proteins in blood serum were assayed using the spectrophotometric method. We have found the increasing of collagenolytic activity by 46.1% (р<0.001), the content of free hydroxyproline by 74.1% (р<0.001), the content of glycosaminoglycans by 1.8 times (р<0.001), the content of fucose, unbound with proteins by 2.8 times (р<0.001) in rats with periodontitis vs. the control group. The development of periodontitis in a setting of thyroid dysfunction leads to an even more significant increase in the destruction of connective tissue, which is confirmed by a significant increase in the content of studied indices vs. euthyroid animals, both in hyperthyroidism and hypothyroidism.

Oxidative processes peculiarity in rats affected by carbon tetrachloride based on malathion intoxication Topicality. The chemical pollution of environment and its impact on the human body is one of the problems not fully studied. It is a question of priority. Carbon and tetrachloride malathion are the most dangerous contaminative elements considered to be the risk factors for many environmentally dependent diseases. Therefore, it is actual to study the characteristics and mechanisms of the aforementioned xenobiotics combined action on the body. Aim. To investigate the free radical processes activity and the endogenous markers of intoxication at the conditions of effect on the rats organism by tetrachloride bbeing affected by malathion. Materials and methods. Experiments conducted on white rats being under carbon tetrachloride intoxication influence (4th, 7th days of the affection) on the basis of thirtieth days malathion affection. Rats were taken out of the experiment by the thiopental anesthesia. Results and discussion. The experimental data proved the malathion and carbon tetrachloride toxic effect, which increases by the conditions of their combined using. Conclusions. It was established, that the maximum activation of free radical processes and deepening endogenous intoxication observed on the seventh day affection by the carbon tetrachloride on on the basis of thirtieth days malathion intoxication.

ДВНЗ «Тернопільський державний медичний університет ім. І. Я. Горбачевського МОЗ України»Особливості перебігу окиснювальних процесів у щурів, уражених тетрахлорометаном на тлі інтоксикації карбофосом Актуальність. Проблема впливу хімічного забруднення навколишнього cередовища на організм людини є однією з пріоритетних та не до кінця вивчених. Серед забруднювачів значну загрозу становлять тетрахло-рометан та карбофос -фактори ризику багатьох екологічнозалежних хвороб. Тому вивчення особливостей та механізмів комбінованої дії вищевказаних ксенобіотиків на організм є актуальним.Метою роботи було дослідити активність вільнорадикальних процесів та маркери ендогенної інтоксикації за умов впливу на організм щурів тетрахлорометану на тлі ураження карбофосом.Матеріали та методи. Експерименти проведені на білих щурах, які піддавались інтоксикації тетрахлоро-метаном (4-та, 7-а доба ураження) на тлі тридцятиденного ураження карбофосом. Щурів виводили з експери-менту під тіопенталовим наркозом.Результати та їх обговорення. Експериментальні дані підтвердили токсичний вплив карбофосу та тетрахлоро-метану, який підвищується за умов їх комбінованого застосування.Висновки. Встановлено, що максимальна активація вільнорадикальних процесів та поглиблення ендоген-ної інтоксикації спостерігається на сьому добу ураження тетрахлорометаном на тлі тридцятиденної інтоксика-ції карбофосом. Particularity of oxidative processes in rats affected by carbon tetrachloride on the background intoxication of malathionTopicality. The problem of the environment chemical pollution impact on the human body is one of the priority and not fully studied. Among the pollutants carbon tetrachloride malathion poses a significant reat -it is a factor of risk for many environmentally dependent diseases. There fore, it is important to study the characteristics and mechanisms of a mentioned xenobiotics combined action on the body .Aim. To investigate the activity of free radical processes and the markers of endogenous intoxication at the conditions of effect on the rats organism by tetrachloride on the background of malathion.Materials and methods. While carrying out an experiment white rats were intoxicated by carbon tetrachloride intoxication (4th, 7th days of the affection) in a period of thirty days malathion affection. Rats were taken out of the experiment by the thiopental anesthesia.Results and discussion. The experimental data proved the malathion and carbontetrachloride toxic effect, which increases the conditions of their combined using.Conclusions. It was established, that the maximum activation of free radical processes and deepening endogenous intoxication observed on the seventh day affection by carbontetrachloride in a period of thirty days malathion intoxication.

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