The experiment was carried out on lab rats which had been given the gluten free nutriment in the conditions of the veterinary clinic of «Saratov State Vavilov Agrarian University». During the research, the morphological and biochemical blood analysis’ results were studied. Laboratory rats’ condition was under daily clinical monitoring as well as their weight. According to the data collected, no external signs of intoxication and death of the rats had been found during the experiment. All rats were active. The concentration of hemoglobin in the experimental group has increased by 14.33g per liter compared to the control group. Therefore, the use of gluten-free nourishment for animals does not have a negative effect on the homeostasis of the organism, which implies the safeness of a gluten-free diet.
The research featured the effect of a diet fortified with essential microelements on the ruminal microbiota of young rams. Ruminal microbiota is largely responsible for feed digestibility and body functioning of cattle.
The study involved the contents of the rumens and jejuna of seven-month-old rams of the Edilbaev breed, which were subjected to a biofortified diet. The diet included the Russian feed additives Yoddar-Zn and DAFS-25 represent a protein-carbohydrate complex with plant silicon. The microflora of the digestive tract was tested using the molecular genetic method of terminal restriction fragment length polymorphism (T-RFLP) sequestration. The microstructural studies of the jejunum samples exploited light microscopy.
The feed additives increased the population of cellulolytic and lactate-fermenting bacteria, as well as the Prevotella sp. microbiome and bifidobacteria in the rumen samples. The data obtained revealed the effect of essential microelements on the taxonomic pattern of microorganisms and the microflora profile. The research revealed the ratio of normal, opportunistic, pathogenic, nonculturable, and transit microflora. The jejunum wall samples obtained from the experimental group that fed on Yoddar-Zn and DAFS-25 had a more distinct micropicture of mucous membrane. Their rumen microflora balance had fewer pathogenic and opportunistic microorganisms, which was also confirmed by the jejunum morphology.
The feed additives DAFS-25 and Yoddar-Zn proved beneficial for ram diet and inhibited the negative effect of pathogenic treponemas on the rumen. The additives improved digestion, absorption, and assimilation of food nutrients, as well as increased the livestock yield.
Studying the morphometric characteristics of gilts’ alimentary canal, no changes in its structure were revealed when having added microelements’ complex on the basis of the L-asparaginic acid into the mixed fodder. According to the results of the conducted research, it was established that 10% of asparaginates are sufficient to provide animals of the 2nd experimental group with higher protective properties.
Влияние аспарагинатов на гомеостаз организма подсвинков РЕЗЮМЕВ большинстве стран мира основное сырье для производства высококачественных мясных продуктов питания -свинина. Ученые считают, что выгодно и экономически целесообразно применение хелатных комплексов в свиноводстве, которые улучшают мясные и откормочные качества животных. С возрастом у большинства животных происходит повышение не только массы тела, но и массы внутренних органов. В наших исследованиях особенно четко просматривается увеличение указанных показателей у подсвинков при добавлении в рационы 10% количества аспарагинатов, что является наиболее оптимальным количеством для подсвинков.
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