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During the investigation of Coichicum plants from Yugoslavia a number of alkaloids of known and unknown structures [1,2,3,4] were isolated but in non of them colchicoside was found. The investigations of BELLET and coworkers [5,6] have shown that the seed of C. autumnale L. from the lairs of Yugoslavia and Italy containes colchicoside. The purpose of this work is both the determination of colchicoside and coichicine and corelation between them, Previous investigations have shown that the seeds contain about 9 % of oil [7] and for that reason the fatty acids were investigated as well.The material used for our investigation were six samples of seed. The oil, coichicine and coichicoside were extracted by EtOH. Etanol extract was destillated and coichicine was extracted by CHC13 [5] and purified by column chromatography [1]. Colchicoside was extracted from the water phase with CH3CI EtOH, 4:1. The fatty acids were separated on a steel column of 3 m lenght, cross section 3,2 mm filled with chromosorb WAW DMCSP 100/250 mesh, with 5 % ethylene alycol succinate. The analysis was performed on a gas chromatograph H. Packard 7620; the detector was the FID 7635 A, and as a gas carrier the He with the flow-rates 25 mi/mm was used. The identification of fatty acids was carried out on the basis of retention times of qualitatively and quantitatively known mixtures of methyl esters of fatty acids. The amount of coichicoside was 0.10-0.25 %, those of colchicine 0.32-0.85 % and both were in correlation. The composition of seed oil was compared with other plants because the data on oil composition of C. autumnale L. seed were not found in literature.

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