This research evaluates the MDA levels, number and apoptosis of spermatogenic cells in Rat provided with duwet fruit extract and exposed to cigarette smoke towards. The study involved 4 experimental animal groups and 6 replications, where K0 was the negative control group, K1 was exposed the cigarette smoke, K2 was given orally duwet fruit, and K3 was given orally duwet fruit after being exposed to cigarette smoke. Therefore, data obtained were analyzed using One way Anova test. The results showed significantly lower number of spermatocytes and MDA levels in the testes in all groups compared to K1 at p <0.05. Spermatogenic cell apoptosis occurred in all groups, and there was also a high tendency for reduction in K2 compared to K1, although not significant at p > 0.05. In addition, apoptosis was expressed by Caspase 3 in the testes. The highest MDA levels found in K1. This phenomenon was assumed to have resulted in lower numbers of spermatocytes. These decreased number of spermatocytes were related with the decline in number of spermatogenic cells prone to apoptosis. In conclusion, duwet fruit had the ability to reduce free radicals with the tendency to inhibit spermatogenesis. This case features an increase in spermatocytes and a potential decline in the incidence of spermatogenic cell apoptosis.

Java Plum fruit can decrease free radical activities. The overwhelmed production of free radicals will reduce the immunity system in the body. This research aims to study the effects of java plum fruit extracts on leukocytes and histopathology of mouse lungs that have been exposed to cigarette smoke for 30 days. Frozen-dried java plum fruit is macerated with water. The mice are divided into Control groups (K-). The mice of the control group were given only water orally. (K+) are mice that are exposed to commercial cigarette smoke once a day. (RJ) were the mice exposed to cigarette smoke once a day as java plum fruit extract orally of 180 mg/kg body weight. (RF) were the mice exposed to cigarette smoke given with java plum fruit extract with 180 mg/kg of body weight that is applied on its filter. The results showed a significantly lower number of leukocytes (p < 0.05) in the K-,RF, and RJ groups compared to K+. Meanwhile, there was a significant increase in the number of lung pathological cells (p < 0.05) in the K+, RF, and RJ compared to the control group (K-). The results conclude that java plum fruit can reduce free radicals in animals affected by inflammation due to cigarette smoke particles in the lungs.