The purpose of this study was to develop Biology learning media with Mixed Reality applications using Pyramid Holograms. This research is a qualitative descriptive study. The research was conducted in two stages, namely the problem identification stage and the development stage. The problem identification stage is done by questionnaire and student test results and the development stage by designing Mixed Reality media. The results of the study were in the form of motion system material media with Pyramid Hologram technology that can be used in the learning process.