This study assessed the aflatoxin B1 (AFB1) intake of the Thai population through consumption of contaminated brown and color rice. A total of 240 rice samples from two harvesting periods were collected in June/July 2012 (period I) and in December 2012/January 2013 (period II) and analyzed for AFB1 by HPLC with fluorescence detection (limit of detection (LOD) = 0.093 ng/g). Exposure assessment was based on AFB1 levels in rice and food intake data for rice according to Thai National Consumption. Frequency and levels of AFB1 were higher in period I (59%,

In-house immunoaffinity column for aflatoxins (AFs) detection is firstly developed by Kasetsart University since 2011 i.e. the first IAC prototype, KU-AF01. Detection of aflatoxin B (AFB ) up to 200 ng is considered significantly 1 1 efficient, but the production cost is considered high. The high cost was due to the cost of monoclonal antibody specific to aflatoxin and its supporting materials i.e. CNBr-activated sepharose 4B. Therefore, this study was aimed to improve the efficiency of in-house immunoaffinity colums by replacing CNBr-activated sepharose 4B with other supporting materials and to lower the ratio of antibody to supporting materials. Response surface methodology (RSM) was applied to determine the optimum alternative supporting materials and the ratio. Results revealed that when compared with CNBr-activated sepharose 4B, all materials tested could recover higher than 80% AFB , except the 1 activated CH sepharose 4B. Results also indicated that the optimum ratio was 0.4 mg anti-aflatoxin monoclonal antibody to 204 µL CNBr-activated sepharose 4B. CNBr-activated sepharose 4B was considered to provide the best precision in recovering AFB . KU-AF02 increased the ability to detect AFB to 500 ppb. The recovery of AFs in the 1 1 reference materials using KU-AF02 was more than 96% successful, with HorRat value range of 0.34 -0.76. The reference materials used were 2 levels of AFs in peanuts, i.e. 47.68 and 72.14 ng/g. In terms of quality control in IAC production, there were no significant differences among the 5 batches produced (p > 0.05). KU-AF02 demonstrated o stable and constant percent recovery at 4 C for up to 12 months (tested with standard 200 ppb AFB ). This study 1 indicated that KU-AF02 could lower the ratio of supporting materials to the antibody and that KU-AF02 has high stability and has good ability for cleaning up AFs. Therefore, KU-AF02 is recommended to be used as an in-house immunoaffinity column for aflatoxin detection in Thailand.