Epstein Barr virus is tumour virus that expresses cancer genes and immortalizes infected lymphocytes. This study is designed to determine the presence of the LMP-1 and human Twist protein and study the association between them in a patient with thyroid cancer and benign lesion. The retrospective study consists of 40 formalin-fixed, paraffin-embedded thyroid tissue blocks were obtained, from 20 patients with thyroid papillary carcinoma and 20 patients with thyroid follicular carcinoma, and compared with 10 tissues from follicular hyperplasia without atypia were collected from Teaching Laboratories in Medical city,  Baghdad, during the period from July 2019 till February 2020. Tissue blocks were used for the detection of LMP1 and human twist protein by immunohistochemistry technique. The results of LMP-1 was (45%, 40% and 30%) respectively and high proportion was noticed in females than males, (77.78%), (78%), (100%) respectively, and the results reveal high percentage 66.67% in age (20-40 years).  Human twist protein shows that the most favourable cases 9 (45%), 8 (40%), in patients with PTC and FTC while no instances in the control group.  In conclusion, LMP1 and human Twist protein may have an essential role in the progression of thyroid cancer.