The quantitative and qualitative analysis of red cell parameters are measured by RBC count, Hematocrit(HCT), Mean Corpuscular Volume(MCV),Mean Corpuscular Hemoglobin(MCH) and Mean Corpuscular Hemoglobin Concentration(MCHC) which gives the indication of red cell deformability and hemorrheological state. The Red

Background: Platelets are also known for their role in the pathophysiology of vascular inflammation. Activated platelets can promote vascular inflammation, causing endothelial inflammation and subsequent leucocyte extravasation. Increased MPV may be used as a marker of platelet activation. We aim to study the relationship of MPV with total WBC count in cases with neutrophilic leucocytosis and to assess if MPV may have any role as an inflammatory marker. Methods:A retrospective study was conducted including 97 cases of leucocytosis with neutrophilia. 95 age and sex matched healthy individuals were used as controls. MPV values and total WBC counts were obtained on automated cell counter Horiba pentra ES60 and manual differential count was performed.Results: MPV did not show any significant change in the study group compared to the control group. MPV was noted to be higher in cases with associated thrombocytopenia, while MPV was lower in cases with thrombocytosis. Conclusion:MPV has no direct role in the evaluation of acute inflammation. However the increased MPV noted in cases of thrombocytopenia with neutrophilic leucocytosis suggests that activation of platelets causes a rise in MPV due to shape change and may be implicated in impending sepsis and disseminated intravascular coagulation due to the production of thromboxane A2 by activated platelets, warranting the need for further workup and prospective studies in order to establish the role of MPV as a marker for impending sepsis and DIC in cases with neutrophilic leucocytosis.

Background: Dengue fever is the most rapidly spreading mosquito-borne viral disease. Four main characteristic manifestations of dengue illness are (i) continuous high fever lasting 2-7 days; (ii) haemorrhagic tendency as shown by a positive tourniquet test, petechiae or epistaxis; (iii) thrombocytopoenia (platelet count <100×109/l); and (iv) evidence of plasma leakage manifested by haemoconcentration (an increase in haematocrit 20% above average for age, sex and population), pleural effusion and ascites, etc. Recently, novel platelet indices such as MPV, PDW, and P-LCR have been investigated as prospective platelet activation markers .Platelet volume, a marker of platelet function and activity is measured as mean platelet volume (MPV) by hematology analyzer. Method:The study was done with the aim to assess whether mean platelet volume has any diagnostic importance in the initial evaluation of dengue fever . 45 adult patients were diagnosed with Dengue NS1 antigen positivity and underwent routine complete blood counts on automated hematology analyser HORIBA Pentra ES60.Result: A total of 76 cases ranging from 6 months to 50 years of age were reported as antigenically positive for dengue indicating acute infection, out of which 45 were adults ranging from 12 to 50 years of age. 34 were male and 11 were female. Infants and children below 12 years of age were not included in this study. 55 controls were studied which included 31 males and 24 females. The mean platelet volume was found to be significantly higher in dengue cases when compared to controls. MPV was also noted to be significantly higher in patients with platelet count below the normal biological reference range of 1.5 lakhs ,when compared to dengue patients with normal platelet counts. Conclusion:To conclude, high MPV indicates platelet activation and may be used as an initial marker to suspect dengue fever in a case of thrombocytopenia.

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Background: With the high prevalence of anemia and increasing incidence of dengue in developing countries like India, diagnosis of anemia in dengue patients needs to be stressed. There is higher hemoglobin and hematocrit as a result of plasma leakage in dengue; hence diagnosis and treatment of anemia may be missed.Methods: A retrospective study was done on 138 dengue patients diagnosed in 2017, in a tertiary care teaching hospital in Hyderabad. The complete blood counts and blood picture data was retrieved and statistical analysis was performed using Microsoft Excel.Result: 60% of patients >5 yrs of age showed low hemoglobin while 93.3% showed hypochromia. In patients aged 5-11yrs and 12-14 yrs, 31.4% and 14.3% respectively had low hemoglobin, but hypochromia was noted in 48.6% and 57.1% .In non pregnant females, 68.4% showed low hemoglobin while 55.3% showed hypochromia. In males, 25% showed low hemoglobin whereas 13.9% showed hypochromia.Wide variability in hemoglobin concentration of same patient was noted with coefficient of variation above 1% in 91.7% of cases. MCV and MCH had coefficient of variation more than 1.1% and 0.5% in 25% and 69.4% of cases respectively. Conclusion:Mean corpuscular volume is a stable parameter which can be used to assess the morphological status of the RBC even in a background of hemoconcentration where the hemoglobin is spuriously raised. The importance of inclusion of red cell indices especially mean corpuscular volume in complete blood counts reports is stressed.