AbstractWe aim to analyze in bounds KJ Tracey’s immunological homunculus conception the relationships between parameters of electroencephalogram (EEG) and heart rate variability (HRV), on the one hand, and the parameters of bhite blood cell count, on the other hand.MethodsIn basal conditions in 23 men, patients with chronic pyelonephritis and cholecystitis in remission, recorded EEG (“NeuroCom Standard”, KhAI Medica, Ukraine) and HRV (“Cardiolab+VSR”, KhAI Medica, Ukraine). In portion of blood counted up white blood cell count.ResultsRevealed that canonical correlation between constellation EEG and HRV parameters form with blood level of leukocytes 0.92 (p<10-5), with relative content in white blood cell count stubnuclear neutrophiles 0.93 (p<10-5), segmentonucleary neutrophiles 0.89 (p<10-3), eosinophiles 0.87 (p=0.003), lymphocytes 0.77 (p<10-3) and with monocytes 0.75 (p=0.003).ConclusionParameters of white blood cell count significantly modulated by electrical activity some structures of central and autonomic nervous systems.

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zukow@umk.pl 4 OO Bohomolets' Institute of Physiology, Kyїv i.popovych@biph.kiev.ua AbstractBackground. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon's entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functionalmetabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.168

Background. Previously, we found a wide range of uric acid exchange parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy rats analyzed. The purpose of this study is to clarify such relationships in patients with neuroendocrine-immune complex dysfunction on the background of chronic pyelonephritis combined with cholecystitis in remission. Material and Methods. The object of observation were 34 men and 10 women aged 24-70 years old, who came to the Truskavets' spa for the rehabilitation treatment. The serum and daily urine levels of the uric acid by uricase method were determined. Immune status evaluated on a set of I and II levels recommended by the WHO. The condition of microbiota is evaluated on the results of sowing of feces and urine. Results. The canonical correlation analysis revealed that raw uricemia determines by 28% nine parameters of immunity (relative blood content of pan-lymphocytes and their CD4 +-, CD56 +-, 0-populations, relative content of polymorphonuclear neutrophils, intensity and completeness of their phagocytosis Staph. aureus and their bactericidal capacity, saliva content of IgG) as well as bacteriuria and content in E. coli feces. Uricemia, normalized by sex and age, determines by 25% another constellation of immunity parameters (relative CD8 + lymphocytes content, CIC, E. coli phagocytosis intensity and completeness, Staph. aureus phagocytosis activity and completeness) as well as content in E. coli feces with impaired enzymatic activity and Klebsiela&Proteus. Instead, uricosuria determines only four parameters of immunity and only by 11,5%. Conclusion. Endogenous uric acid has a modulating overall beneficial effect on a number of immune and microbiota parameters in both healthy rats and people with neuroendocrine-immune complex dysfunction.