Research ArticleHPLC analysis, anti HPLC analysis, anti HPLC analysis, anti---oxidant activity oxidant activity oxidant activity of of of Genista ferox Genista ferox Genista ferox and its anti and its anti and its anti---proliferative effect proliferative effect proliferative effect in in in HeLa HeLa HeLa cell cell cell line line line BJP IntroductionPlants used in ancient times as medicines to alleviate symptoms of various ailments (Saeed et al., 2012). In spite of the great progress in modern medicine in recent decades, but the herbal medicine still make an important contribution in the health care. Many medicinal and aromatic plants contain huge amounts of antioxidants such as polyphenols. These substances can have an important role in the absorption and neutralize free radicals, and the extinction of the shirt and triple oxygen or peroxide decomposing. Many of these phytochemicals have anti-oxidant capabilities so they contribute significantly to the fight against many human diseases and thus contribute to the reduction of mortality (Djeridane et al., 2006). Phenolic compounds such as flavonoids and phenolic acids have different biological effects, such as the effects of anti-atherosclerotic, anti-inflammatory and anti-cancer, as a result of the anti-oxidant activity (Krishnaiah et al., 2011).The Fabaceae family contains approximately 700 genera, in Algeria there are about 53 genera and 337 species (Quezel and Santa, 1963). Genista genus has about 150 species in Europe and the Mediterranean region (RE, 1987). A literature survey shows that this genus is a good source of phenolic compounds, in particular isoflavonoids, which are known for their diverse biological activities. The recent studies on the species of the Genista genus showed pharmacological interest (Belle et al., 1995;Herrera et al., 1992).In the present study, the qualitative and quantitative analysis, the identification and quantification of phenolic acids and flavonoids in chloroform, ethyl AbstractThe prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant activity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by Folin-Ciocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 -308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 -124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against H...