The current study provides a detailed qualitative description of the organization of the cholinergic, catecholaminergic, serotonergic, orexinergic, and GABAergic sleep‐related systems in the brain of the blue wildebeest (Connocheates taurinus), along with a quantitative analysis of the pontine cholinergic and noradrenergic neurons, and the hypothalamic orexinergic neurons. The aim of this study was to compare the nuclear organization of these systems to other mammalian species and specifically that reported for other Cetartiodactyla. In the brain of the blue wildebeest, from the basal forebrain to the pons, the nuclear organization of the cholinergic, catecholaminergic, serotonergic, and orexinergic systems, for the most part, showed a corresponding nuclear organization to that reported in other mammals and more specifically the Cetartiodactyla. Furthermore, the description and distribution of the GABAergic system, which was examined through immunostaining for the calcium binding proteins calbindin, calretinin, and parvalbumin, was also similar to that seen in other mammals. These findings indicate that sleep in the blue wildebeest is likely to show typically mammalian features in terms of the global brain activity of the generally recognized sleep states of mammals, but Cetartiodactyl‐specific features of the orexinergic system may act to lower overall daily total sleep time in relation to similar sized non‐Cetartiodactyl mammals. Anat Rec, 2019. © 2019 American Association for Anatomy Anat Rec, 303:1977–1997, 2020. © 2019 American Association for Anatomy

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Employing orexin-A immunohistochemistry, we describe the distribution, morphology, and nuclear parcellation of orexinergic neurons within the hypothalami of an Asiatic lion (Panthera leo subsp. persica), an African lion (Panthera leo subsp. melanochaita), and a Southeast African cheetah (Acinonyx jubatus subsp. jubatus). In all three felids, the clustering of large, bipolar, and multipolar hypothalamic orexinergic neurons primarily follows the pattern observed in other mammals. The orexinergic neurons were found, primarily, to form three distinct clusters-the main, zona incerta, and optic tract clusters. In addition, large orexinergic neurons were observed in the ventromedial supraoptic region of the hypothalamus, where they are not typically observed in other species. As has been observed in cetartiodactyls and the African elephant, a cluster of small, multipolar orexinergic neurons, the parvocellular cluster, was observed in the medial zone of the hypothalamus in all three felids, although this parvocellular cluster has not been reported in other carnivores. In both subspecies of lions, but not the cheetah, potential orexin-immunopositive neurons were observed in the paraventricular hypothalamic nucleus, supraoptic nucleus, the lateral part of the retrochiasmatic area, and the inner layer of the median eminence. The distribution and parcellation of orexinergic neurons in the hypothalami of the three felids studied appear to be more complex than observed in many other mammals and for the two subspecies of lion may be even more complex. These findings are discussed in terms of potential technical concerns, phylogenetic variations of this system, and potentially associated functional aspects of the orexinergic system.

Rest is a state of adaptive inactivity that increases the efficiency of activity by regulating its timing and reducing energy use when activity is not beneficial. Thus, animals can go without rest when specific demands, such as mating, favour being awake. Sexually active male blue wildebeest (bulls) are typically territorial, and it has been reported that when a bull is protecting a harem during the mating season (rut), he neither eats nor rests. We examined the daily activity and inactivity patterns of dominant bulls by means of actigraphy for 3 months, which included the rut. We also measured faecal androgen metabolite (fAM) levels and subcutaneous temperature, both of which have variances known to delineate the rut. During the rut, wildebeest bulls experienced higher levels of activity, fAM, and a greater daily range of subcutaneous temperature. Despite previous reports, the male blue wildebeest rested daily during the rut, and while the amount of rest was low, it was not substantially lower than prior to the rut. The amount of time spent inactive increased substantially after the rut. The timing of daily activity and inactivity patterns did not vary substantially across the recording period. Across the recording period, the average daily ambient temperatures decreased (seasonality), and the subcutaneous temperature followed this pattern, although it was not as marked. It appears that in the post-rut period a substantive increase in time spent at rest occurs, potentially allowing the wildebeest bulls time to recover following a period of intense activity.

The current study provides an analysis of the cholinergic, catecholaminergic, serotonergic, and orexinergic neuronal populations, or nuclei, in the brain of the lesser hedgehog tenrec, as revealed with immunohistochemical techniques.