IntroductionKeloids characterized by fibroblast hyperproliferation and depositions of collagen which similar to cancer cells. Tagitinin C is a class of sesquiterpene lactones (SLS) was isolated from the leaves of the moon flower Tithonia diversifolia (Hemsley) A. Gray. The study aim is to evaluate the effects of tagitinin C from Tithonia diversifolia to keloid fibroblasts (KF).MethodsMonolayer cultures of keloid fibroblast (three passages) were treated with 8 serial concentration of tagitinin C (0.015 to 2) μg/mL during 72 and 120 hours. A positive control using mitomycin C. Cellular viabilities were measured by MTT assay. Collagen depositions were measured by Sirius Red assay for nonsoluble collagen.ResultsThe reading of the result was conducted by ELISA reader. Data were analyzed by probit regression with SPSS 19 for Windows. The result showed that tagitinin C can inhibit keloid fibroblasts (KF) viability with IC50 0.122 μg/mL (incubation 72h) and 0.039 μg/mL (120h), whereas mitomycin C IC50 0.120 μg/mL (72h) and IC50 of 0.100 μg/mL (120h). At IC50 concentration of tagitinin C on keloid collagen deposition 53.1% (72h) and 44.3% (120h), whereas the IC50 concentration of mitomycin C on keloid collagen deposition 60.4% (72h) and 52.1% (120h). Selectivity index tagitinin C on normal fibroblasts (NF) is 287 for 72h incubation and 791 for 120h incubation.ConclusionIt can be concluded that the ability of tagitinin C inhibits KF viability and decreasing keloid collagen deposition is consistent with the concentration (concentration-dependent) and incubation time (time-dependent). Tagitinin C has a low toxicity level on NF with high selectivity index.

Background: Kersen leaf has the potential to lower triglycerides because they contain flavonoids and tannins. Methods: This study was an experimental laboratory study with a pre and post-test with a control group design. The subjects were 30 white male rats (Rattus norvegicus), bodyweight ±200 grams, aged 3-4 months, and divided into 5 groups with random sampling. The groups were negative control (K-) was given a high-fat diet, and PTU without any treatment, positive control (K+) was given a high-fat diet, and PTU with gemfibrozil treatment, (P1),(P2),(P3) was given a high-fat diet and PTU with ethanol extract of Kersen leaf 100, 200, and 400 mg/kgBB. This study was held for 4 weeks. Triglyceride level was measured using the GPO-PAP method. The data was analyzed using Shapiro-Wilk, Levene test, and ANOVA test. Results: The ethanol extract of Kersen leaf dose 100, 200 and 400 mg/kgWB are found effective on reducing blood triglyceride levels with a mean reduction in the first treatment of 112,83 mg/dl, 98,20 mg/dl, 89,92 mg/dl and in the second treatment of 106,71 mg/dl, 93,95 mg/dl dan 76,87 mg/dl. Statistical analysis using one-way ANOVA showed p<0,05, which determines a significant difference in triglyceride levels among each group. Conclusion: Extract dose of 400 mg/kgWB is found to be the most effective dose in triglyceride reduction.

Typhoid fever is an infectious disease caused by Salmonella typhi bacteria. The administration of antibiotics in typhoid fever is needed as a causative therapy to eradicate the bacteria based on the principles of rational therapy to avoid antibiotic resistance. However, there are still a considerable amount of cases of unnecessary antibiotic administration. This study aims to examine the rationality of antibiotic use on patients of typhoid fever. This research is a non-intervention study with descriptive analytic methods. Data were retrieved retrospectively by identifying the medical records of patients treated in the X Hospital Salatiga from January to August 2016 with a purposive sampling method based on the inclusion and exclusion criteria of 67 cases. The data was analyzed by using Gyssen criteria based on the standard of typhoid fever service of WHO 2011. The result showed that the percentage of female typhoid fever patients was higher than the male with the most prolonged 3-day hospitality. The most commonly used antibiotics are ceftriaxone, ciprofloxacin, and cefixime with the result of the rationality analysis of category 0 (55.22 %), category IIIA (1.49%), category IIIB ( 8.96% ), category IVA (17.91%), and category IVC (4.48%). It can be concluded that the rationality of antibiotics used in patients with typhoid fever at X Hospital Salatiga based on Gyssen criteria showed a good result.

Abstrak: Sindrom metabolik merupakan kumpulan gangguan metabolik yang dapat meningkatkan angka kesakitan dan kematian, oleh karena itu diperlukan pemahaman dan kesadaran masyarakat untuk mencegah penyakit sindrom metabolik. Kegiatan pengabdian kepada masyarakat (PKM) ini bertujuan memberikan edukasi dan pemeriksaan skrining untuk mencegah sindrom metabolik. Peserta PKM ini adalah ibu-ibu PKK Karangjati RT.07 Bangunjiwo Bantul Yogyakarta berjumlah 40 orang. Kegiatan PKM meliputi senam bersama, edukasi, serta skrining sindrom metabolik. Dari 40 orang peserta PKM diketahui 20 orang memiliki risiko tinggi mengalami sindrom metabolik yang ditandai dengan adanya obesitas, peningkatan tekanan darah, hiperkolesterolemia, dan hiperglikemia. Hasil nilai pre-test dan post-test menunjukkan adanya peningkatan pemahaman dari 7,5% menjadi 85%peserta PKM tentang sindrom metabolik.Abstract: Metabolic syndrome is a collection of metabolic disorders that can increase morbidity and mortality, therefore public understanding and awareness is needed to prevent metabolic syndrome. This community service activity (PKM) aims to provide education and screening tests to prevent metabolic syndrome. The PKM participants are PKK Karangjati women RT.07 Bangunjiwo Bantul Yogyakarta totaling 40 people. PKM activities include body exercise, education, and screening examinations. There were 20 PKM participants who had a high risk of experiencing metabolic syndrome which was characterized by obesity, increased blood pressure, hypercholesterolemia, and hyperglycemia. The results of the analysis of pretest and posttest scores according to educational materials showed an increase in the understanding of PKM participants about the metabolic syndrome.

Non-communicable Diseases (NCDs) are rarely detected early because they are usually asymptomatic or without complaints. Some patients are usually found in an advanced stage so difficult to cure and end up with illness or premature death. One of the efforts to control PTM is to empower and increase community participation through NCDs posbindu activities with their cadres. The purpose of this Community Counseling (PKM) is to provide education and training about non-communicable diseases to health cadres in the working area of the Piyungan Health Center as partners in this PKM. This PKM uses the Active Person Learning Method (CBIA) which consists of 2 activities, namely counseling containing NCDs materials and training for NCDs early detection skills. This activity was attended by 30 cadres who were very enthusiastic during the activity. The results of the activity showed an increase in the knowledge of cadres in the good category of 80%, and 20% sufficient. The improvement of cadre skills showed good results, namely 100% of cadres were able to check vital signs and anthropometry and 70% of cadres were able to check blood sugar levels. This PKM activity was concluded to be able to increase the knowledge and skills of cadres about NCDs.