BACKGROUND Environmental concerns are driving the call for adoption of alternative nonchemical weeding approaches. This study aimed to develop and evaluate a novel, precise, low‐energy electrophysical treatment weeding systems and to provide new insight into their control mechanism. Two electrophysical treatment systems, based on AC (2.2 kV) and DC (40 kV) energy sources, were developed and evaluated. The impacts of various operational and biological factors on the weed control effectiveness were evaluated. Additionally, thermal images were taken during the treatments to document plant temperature. RESULTS Treatments via direct leaf contact caused greater damage to Amaranth plants than the stem contact treatments, with 75% and 20% biomass reduction compared to control, respectively. Treatment of early growth stages was favorable over later growth stages, with 100% and 75% biomass reduction for Trifolium pretense plants treated with 0.0125 W h 2 and 4 weeks after seeding, respectively, compared to control. Additionally, the applied energy affected treatment performance, with its impact varying across the growth stages and species; at the two‐leaf growth stage, 0.0025 W h treatment was sufficient for plant death. A >40 °C increase in plant temperature was measured during the electrophysical treatment, with the temperature of some plant organs reaching ~70 °C. CONCLUSION Results from this study demonstrate the potential use of electrophysical treatment as an effective weed control tool. The low energetic demands in the new systems provide suitable control results when applied at early stages. Temperature increase seemed to be one of the main control factors, yet efficacy was affected by various biological factors.