Research on the diabetes mellitus (DM) treatment has recently been carried out, especially on discovering antidiabetic agents derived from plants. This study aims to determine the fabrication of microzone plastic-based biosensors, determine the optimal sensor conditions, analysis characteristics, and compare the biosensor with the UV-Vis spectrophotometric method. The results showed that the response time was 15 minutes, the linear range was 500-40000 µg / mL (R = 0.9989). The limit of detection (LOD) was 1109.6 µg / mL, and the limit of quantification (LOQ) was 3698.8 µg / mL. Biosensor filled up the precision parameters with an RSD value of less than 3.7% and an accuracy with recovery in the range of 95-105%. The biosensor is stable in storage at 25 ° C for 270 minutes and at chiller temperature for three days. The antidiabetic activity of the biosensor was compared with the UV-Vis spectrophotometer using the Independent Sample T-test and showed insignificant differences between the two methods.