Background. Previously, we found a wide range of uric acid exchange parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy rats analyzed. The purpose of this study is to clarify such relationships in patients with neuroendocrine-immune complex dysfunction on the background of chronic pyelonephritis combined with cholecystitis in remission. Material and Methods. The object of observation were 34 men and 10 women aged 24-70 years old, who came to the Truskavets' spa for the rehabilitation treatment. The serum and daily urine levels of the uric acid by uricase method were determined. Immune status evaluated on a set of I and II levels recommended by the WHO. The condition of microbiota is evaluated on the results of sowing of feces and urine. Results. The canonical correlation analysis revealed that raw uricemia determines by 28% nine parameters of immunity (relative blood content of pan-lymphocytes and their CD4 +-, CD56 +-, 0-populations, relative content of polymorphonuclear neutrophils, intensity and completeness of their phagocytosis Staph. aureus and their bactericidal capacity, saliva content of IgG) as well as bacteriuria and content in E. coli feces. Uricemia, normalized by sex and age, determines by 25% another constellation of immunity parameters (relative CD8 + lymphocytes content, CIC, E. coli phagocytosis intensity and completeness, Staph. aureus phagocytosis activity and completeness) as well as content in E. coli feces with impaired enzymatic activity and Klebsiela&Proteus. Instead, uricosuria determines only four parameters of immunity and only by 11,5%. Conclusion. Endogenous uric acid has a modulating overall beneficial effect on a number of immune and microbiota parameters in both healthy rats and people with neuroendocrine-immune complex dysfunction.

zukow@umk.pl 4 OO Bohomolets' Institute of Physiology, Kyїv i.popovych@biph.kiev.ua AbstractBackground. Previously, we found a wide range of uric acid metabolism parameters and functional relationships of uricemia and uricosuria with the parameters of immunity in healthy female rats analyzed. It was found by canonical correlation analysis that both parameters of uric acid metabolism determine the immunity status of rats by 71%. The purpose of this study is to determine the features of immune status in rats with quantitatively and qualitatively different uric acid metabolism. Material and Methods. Experiment was performed on 60 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum. The rats of others groups for 6 days administered through the tube various fluids. The serum and urine levels of the uric acid (uricase method) were determined. In the blood, the parameters of immunity were determined. From thymus and spleen made smears-imprints for counting splenocytogram and thymocytogram. For them, as well as immunocytogram and leukocytogram of blood, Shannon's entropy was calculated. Results. Screening of immune profiles of four quantitative and qualitative uric acid metabolism clusters revealed 6 functionalmetabolic patterns, two of which are quasilinear (enhancing and suppressing), with immune extremes at moderately elevated uricemia, two U-shaped with immune optimum at moderately reduced uricemia levels, as well as a non-reactive pattern. Discriminant analysis revealed 14 parameters of immunity, the constellation of which four clusters of metabolism of uric acid clearly distinguish. Conclusion. Endogenous uric acid exerts a modulatory immunotropic effect in healthy female rats.168

Relationships between changes in uric acid parameters metabolism and parameters of immunity and microbiota in patients with neuroendocrine-immune complex dysfunction.

Background. Uric acid is traditionally considered as the final product of human and primate DNA/RNA degradation, devoid of useful physiological activity. However, there is an opinion that the uric acid molecule, by analogy with methylxanthines (caffeine, theophylin, theobromine) has physiological activity. The acquired experience allows us to offer a topic for future research: "Neurotropic and immunotropic activity of endogenous uric acid". Working hypothesis. Uric acid, interacting with A1 and A2a adenosine receptors as well as phosphodiesterase and Na,K-ATPase of neurons, modulates the activity of nerve centers, which in turn modulate immunocytes. Perhaps the direct effect of uric acid on immunocytes, since the existence of theophylline-resistant and theophylline-sensitive subpopulations of Tlymphocytes has long been known. The proposed article is the first swallow of the announced project. Material and Methods. Experiment was performed on 58 healthy female Wistar rats 220-300 g. Among them 10 animals remained intact, using tap water from drinking ad libitum.The rats of others groups for 6 days administered through the tube various fluids at a dose of 1,5 mL/100 g of body mass. The day after the completion of the drinking course the plasma

Background. Neonatal hyperbilirubinemia has been known to damage neural function. Our goal is to determine whether the neurotropic activity of normal bilirubinemia in adults is evident. Methods. The object of observation were 77 volunteers: 30 women and 47 men aged 49±13 (26 ÷ 76) years without clinical diagnosis. Testing was performed twice with an interval of 4 ÷ 10 days. We determined the plasma levels of the direct and free bilirubin, recorded EEG and HRV followed by analysis of correlations between parameters. Results. Significant downregulating effect of bilirubinemia was found on power spectrum density (PSD) theta and delta rhythm. In contrast, bilirubinemia has an upregulating effect on PSD beta rhythm and sympatho-vagal balance. The canonical correlation between direct & free bilirubin levels, on the one hand, and EEG & HRV parameters, on the other hand, is very strong: R=0,808; R2=0,654; χ2(80)=191; p<10-6 (n=154). A similar canonical correlation was found between individual changes in parameters: R=0,753; R2=0,568; χ2(48)=83; p=0,001 (n=74). Conclusion. Even normal bilirubinemia has an downregulating effect on mainly theta and delta rhythm-generating nuclei and vagal tone, while upregulating effects on sympathetic tone and beta rhythm-generating nuclei.