Flow cytometry with its numerous advantages, has become indispensable for modern biochemistry research, being a highly efficient and sensitive in many areas, particularly in the fundamental or applied cellular biology. Flow cytometry has greatly contributed to the exploration of the cellular structure and functions, combining analytical and preparative aspects. In combination with the use of the probes (fluorochromes, fluorogenic substrates, monoclonal antibodies and lectins), the FCM enables multiple investigations for the characterization of cellular populations, the analysis of the cellular components (nucleic acids, proteins, intercellular and surface antigens, enzyme activities, membrane potential, mitochondrial activity, intracellular pH, ion flux and membrane fluidity). We chose to study the behavior of fibroblasts using flow cytometry, in terms of effects induced by the presence of extracts from Trigonella foenum graecum L.