Nowadays, in the era of 4 th Industrial Revolution, businesses have to face many challenges. The purpose of this study is to identify the readiness level of Greek businesses to face the challenges of Industry 4.0. A questionnaire survey was conducted to Greek business operating in various industries. The paper contributes to identify whether businesses take into consideration various aspects of Industry 4.0, if they are willing to adopt new digital technologies and the kind of difficulties that they face. This study covers the time period from before the economic crisis, and it is proposed that a new survey should be conducted considering the addressed topics regarding the current economic situation.