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The paper is devoted to studying the stability of of dispersed systems (DS) of nanoparticles (NP) of nickel (nNi), platinum (nPt), titanium dioxide (nTiO2), zinc oxide, multicomponent nanopowder (MNP) and multi-walled carbon nanotubes (MWCNT), formed basing on the growth media used in bioassay with application of aquatics: bacterial test-cultures Ecolum, Photobacterium phosphoreum and Escherihia coli, unicellular algae Chlorella vulgaris Beijer, infusoria Paramecium caudatum, cladocera Daphnia magna Straus and Ceriodaphnia affinis, fish Danio rerio. In the process of establishing a test-system the increase in the initial nanosubstance concentration of more than 5 mg/l results in the increase in the speed of aggregation of NP into DS and the decrease in their final concentration. We suggest the variants of estimation of effective concentration of NP in long-term biotesting.

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