The article shows that the smart city management systems requires continuous monitoring of environmental parameters (air, soil and water quality, temperature, humidity, toxic impurities, etc.) and the placement of a large number of different sensors, among which biosensors occupy a leading position. The study analyzed patent documents for leading countries of the world (Great Britain, France, Japan, Germany, USA, Russia) and it showed a steady increase in the number of patents on biosensors. The reasons for the growth and continuous expansion of the scope of biosensors are: cost reduction, increased durability, speed, reliability, accuracy and sensitivity; increase in their functionality. The article presents a classification of biosensors indicating the percentage of patents by type of transducer and the number of patents by bioselective elements, as well as a description of an automated system for the synthesis of new biosensor designs based on the energy-information method. Paper is given the resulting solution can be used as a skeletal structure to create new biosensors, or improved using the knowledge base on techniques for improving the technical and operational characteristics of physical and technical effects.