No abstract

There are numerous studies of multiple antigen-antibody systems employing the technique of localization and separation of specific precipitates in a gel matrix (1-6). Attempts to correlate such information with that obtainable by other techniques, such as the quantitative precipitin method (cf. reference 7), are, however, few (6, 8). The studies described below are an attempt to provide this correlation by investigating relatively pure protein and carbohydrate antigens by gel diffusion methods and the quantitative precipitin technique and applying the results to a relatively impure system, diphtheria toxinantitoxin.