We have cloned a novel human autoimmune antigen in a patient suffering from rheumatoid arthritis with high levels of antibodies to the nucleolus organizer regions. Initially the human autoimmune serum was used to select a cDNA of 317 amino acids from a hamster expression library. Using the hamster DNA as a probe, we isolated the human homologous cDNA of 320 amino acids. Human and hamster polypeptides share a 95% amino acid homology. The deduced 36-kDa protein contains a putative amino-terminal NLS signal, nine cysteine-X-X-cysteine motifs highly conserved, and a carboxyl-terminal poly acidic region. Several homologous expressed sequence tags have been identified in data bases suggesting that orthologous proteins are present throughout evolution from worms to humans. A Drosophila expressed sequence tag was further completely sequenced for a full-length protein with 60% amino acid identity to the human homologue. Northern blot analysis revealed that this novel protein is widely distributed in human tissues with significantly higher expression levels in heart and skeletal muscle. Specific antibodies to the recombinant protein and transfection experiments demonstrated by immunofluorescence the localization of the protein predominantly but not exclusively to the nucleolus of interphase mammalian cells. In actinomycin D-treated cells the protein remains associated with the nucleolus but is not segregated, like other ribosomal factors such as upstream binding factor. In mitosis the protein was found to be associated with centromeres and concentrated at the midbody in cytokinesis. Transient distribution of this evolutionarily conserved zinc finger nucleolar autoantigen to the mitotic centromeres may provide the means for several aspects of cell cycle control and transcriptional regulation.

PURPOSETo develop a scale for a group of symptoms based on the unemployment syndrome.METHODSThe development consisted of four phases: (I) review of literature, (II) concept elicitation, (III) concept selection and item construction, and (IV) content validation.FINDINGSTwenty final items that were established as adequate for being included in the instrument.CONCLUSIONThe unemployment syndrome scale could be used to identify the needs and sintomatology of unemployed people and support the identification of the syndrome.IMPLICATIONS FOR NURSING PRACTICEThe instrument makes it possible to assess the symptomatology specific to the syndrome and to record properly the assessment of the biopsychosocial conditions on the part of the nursing profession for providing quality comprehensive care.