Introduction: Anxiety is considered one of the most disabling diseases and possibly by 2030 it will be the second largest disease worldwide. Research shows that some medicinal plants can be used to treat anxiety y and depression because they act in the central nervous system (CNS). Objective: To evaluate the production profile of herbal medicines for the treatment of anxiety y and depression by Brazilian pharmaceutical industries. Method: This is a quantitative research on pharmaceutical laboratories associated with SINDUSFARMA that produce herbal medicines for anxiety and depression. Result: It was possible to identify that of the 220 pharmaceutical laboratories operating in the country, only 29 manufacturing units produce herbal medicines, corresponding to 14.35%. Conclusion: The Brazilian pharmaceutical industries that produce herbal medicines focused on anxiety and depression are aligned with the findings in the literature review. Brazil is a country with a very rich biodiversity, however it has such a small share in the production of active inputs for the herbal industries, as well as in the production of herbal medicines.

Introduction: Lauraceae alkaloids are a structurally diverse class of plant specialised secondary metabolites that play an important role in modern pharmacotherapy, being useful as well as model compounds for the development of synthetic analogues. However, alkaloids characterisation is challenging due to low concentrations, the complexity of plant extracts, and long processes for accurate structural determinations. Objective: The use of high-performance thin layer chromatography coupled with desorption electrospray ionisation multistage mass spectrometry (HPTLC DESI-MS n) as a fast tool to identify alkaloids present in Ocotea spixiana extract and evaluate the extract's acaricide activity. Methods: Ocotea spixiana twigs were extracted by conventional liquid-liquid partitioning. HPTLC analysis of the ethyl acetate extract was performed to separate isobaric alkaloids prior to DESI-MS n analysis, performed from MS 3 up to MS 7. The extract's acaricide activity against Rhipicephalus microplus was evaluated by in vitro (larval immersion test) and in silico tests. Results: HPTLC-DESI-MS n analysis was performed to identify a total of 13 aporphine and four benzylisoquinoline-type alkaloids reported for the first time in O. spixiana. In vitro evaluation of the extract and the alkaloid boldine showed significant activity against R. microplus larvae. It was established in silico that boldine had important intermolecular interactions with R. microplus acetylcholinesterase enzyme. Conclusion: The present study demonstrated that HPTLC-DESI-MS n is a useful analytical tool to identify isoquinoline alkaloids in plant extracts. The acaricide activity of the O. spixiana ethyl acetate extract can be correlated to the presence of alkaloids. K E Y W O R D S acaricide activity, alkaloids, HPTLC-DESI-MS n , Ocotea spixiana 1 | INTRODUCTION Ocotea is an expressive genus of the Lauraceae family, occurring mainly in tropical and subtropical areas. 1 Species of this genus are known for producing a variety of secondary metabolites, with neolignans, aporphine and benzylisoquinoline-type alkaloids as the most representative classes. 2,3 A wide range of biological activities from Ocotea species has been described in the literature, including

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