Chemical investigation was carried out to examine the risk assessment, dissipation behavior, persistence, and half-life period of quinalphos in/on green pea fruit by spraying quinalphos at fruiting stage followed by another application after 10-day interval. The samples were extracted by using the quick, easy, cheap, effective, rugged, and safe method, and the residues of quinalphos were analyzed by gas chromatography with electron capture detector. Herein, we report a novel, accurate, and cost-effective gas chromatography method for the determination of average deposits of quinalphos in/on green pea. The initial deposits and half-life of quinalphos were found to be 1.20 mg/kg and 2.77 days, respectively, following the application of insecticide. Residues of quinalphos reached below detection limit of 0.05 mg/kg after 10 days at recommended dosage. For risk assessment studies, the tenth day will be safe for consumers for consumption of green pea. The developed method is simple, selective, and repeatable, and it can be extended for quinalphos-based standardization of herbal formulations containing green pea and its use in pesticide industries.

Residue investigation was carried out to scrutinize the persistence, dissipation behavior, half-life, and risk assessment of ethion on green pea fruit by spraying ethion at the fruiting stage followed by another application at 10 day intervals. The samples were extracted by using a quick, easy, low-cost, effective, rugged, and safe method, and the residues of ethion were analyzed by gas chromatography with electron capture detection. Here we report a novel, accurate, and cost-effective gas chromatography method for the determination of average deposits of ethion on green pea. The initial deposits were found to be 4.65 mg/kg following the application of insecticide. Residues of ethion reached below the detection limit of 0.10 mg/kg after 25 days at recommended dosage. The half-life of ethion was found to be 4.62 days. For risk assessment studies, the 25th day will be safe for consumers for the consumption of green peas. The developed method is simple, sensitive, selective, and repeatable and can be extended for ethion-based standardization of herbal formulations containing green pea and its use in pesticide industries.

BACKGROUND The increasing and extensive use of pesticides worldwide has resulted in a significant loss of non‐target populations particularly humans by direct or indirect exposures. Also, various methods have been used for the estimation of pesticide residues in fruits and vegetables from recent past which are either tedious, time consuming or expensive. Therefore, the present study was performed to determine the pesticide residues from apple by simple and novel validated gas chromatography. RESULTS A novel, accurate, ecofriendly and cost‐effective gas chromatography method was developed for simultaneous quantification of eight pesticides, namely chlorpyrifos‐methyl (1), chlorpyrifos (2), quinolphos (3), profenofos (4), myclobutnil (5), ethion (6), fenpropathrin (7) and cypermethrin (8). The developed method was validated as per the SANTE guidelines. All calibration curves showed a good linear relationship (r > 0.99) within the test range. Precision was evaluated by intra‐ and inter‐day tests with relative standard deviations (RSDs) < 2.0%, recovery in between 70% and 120% with RSDs < 2.00%. CONCLUSION The results demonstrate that the concentration of pesticides 1 to 8 were found below the detectable limit. Method validation parameters like linearity, precision, accuracy, specificity, robustness, detection and quantification limits were found within the acceptable range. The proposed method makes it possible to determine simultaneously pesticides 1–8 in one run which can be extended for residue‐based standardization of pesticides from apple and other fruits and vegetables. © 2019 Society of Chemical Industry

The present work describes the persistence, dissipation behaviour, half‐life, risk assessment and novel gas chromatography method for the residue estimation of cypermethrin in green pea by spraying cypermethrin 10EC at 50 g a.i. ha−1 at fruiting stage followed by another application at a 10 day interval. The sample extraction and cleanup was followed bya modified quick, easy, cheap, effective, rugged, and safe method, and the residues of cypermethrin were determined using a validated gas chromatography method. The initial deposits were found to be 1.21 mg kg−1 following the application of insecticide at 50 g a.i. ha−1. Cypermethrin residues declined to below the detection limit of 0.05 mg kg−1 after 15 days at the recommended dosage. The half‐life of cypermethrin was 2.66 days at 50 g a.i. ha−1. For risk assessment studies, the waiting period of 15 days is recommended as safe for consumption for the insecticide. The GC‐ECD method was validated according to the SANTE guidelines by various analytical parameters including linearity, accuracy, detection and quantification limits. The developed method is simple, selective and repeatable, and can be used for the standardization of pesticides on fruits and vegetables.

Herein, the simultaneous quantification, dissipation, risk assessment, half‐life period and method validation of carbendazim and triazophos by high‐performance liquid chromatography has been described. The developed method was validated as per the sante guidelines 2017. All calibration curves showed a good linear relationship (r > 0.99) within test range. Precision was assessed by intra‐ and inter‐day tests with relative standard deviations less than 5%. Recovery was observed within acceptable limit. The residue level of carbendazim and triazophos residues were observed upto the third day and reached below the detection limit on green pea on the fifth day after treatment with carbendazim and triazophos formulation at recommended dosages. Based on the findings, waiting period of 5 days is recommended for consumption of green pea. The presented study would be helpful for safe usage of carbendazim and triazophos on green pea and to prevent any health risk to consumers. The developed method is simple, easy, sensitive and repeatable with a short run time, and can be extended for residues based standardisation of pesticide formulations containing green pea and its use in pesticide industries.