This Clothing is placed not exclusively to protect the human body from harm, but also because it assists in achieving a state of comfort during strenuous activities or when the environment around the wearer is unfavorable. Clothing comfort is determined by the thermo-physiological comfort properties of the woven fabric, which include air permeability, thermal properties, moisture management, and handling properties. Different high-tech fibers, yarns, woven fabric construction and structure have radically altered people's fundamental need to be clothed as time has progressed. In this study, the thermo-physiological comfort properties of woven fabrics with differing construction and structure are investigated. Then comparisons are made keeping all parameters constant except one. It has been demonstrated that a higher thermal conductivity can be achieved by using coarser yarn in higher interlacing fabrics, such as plain fabric. On the other hand, the thermal conductivity of a fabric with low interlace density, such as 3/1 twill, is reduced when a coarser yarn is used. The thermal conductivity of fabric can be reduced to an acceptable level by using coarser yarn and less interlacing. Fabrics with heavier constructions are less air permeable than those with lighter constructions.