Interactive multimedia is a learning medium that presents the material with a combination of text, images, audio, video, and animation. The purpose of this research and development is to produce an interactive multimedia product that is valid, feasible, and effective for learning activities on the human circulatory system material at the elementary school level. The method used in this research is Research and Development (R&D) with the Lee & Owens development model consists of 5 stages as follows: (1) analysis, (2) planning, (3) development, (4) implementation, (5) evaluation. The validation stage was carried out on media experts and material experts and then tested on 19 grade V students of SDN Tamiajeng. The results of interactive multimedia development were declared valid with the percentage of media experts was 98.61%, 79.16% of material experts, 82.98% small group trials, 84.36% large group trials. Interactive multimedia is declared effective for learning by obtaining a percentage of 84.21%. Because of this finding, it can be concluded that interactive multimedia material on the human circulatory system produces valid, feasible, and effective categories to be used for learning.