Trichomoniasis is a common sexually transmitted disease (STD) caused by Trichomonas vaginalis. The current study was conducted during the period from January 2022 to July 2022 to investigate the infection of Trichomonas vaginalis  in Iraqi women at Wasit province . A total of 100 women of different ages were enrolled in this study. The vaginal swabs were collected from each woman. A consent form which included age, area of residence, educational level , in addition to the symptoms were obtained from the participants. By Wet mount examination , 4% infection rate with Trichomonas vaginalis. The age class of 20-30 years had the highest rate of infection (3%); while infection among patients from urban resident (3%) was higher than of rural resident (1%). Women with primary educational level showed higher prevalence of Trichomonas vaginalis (2%) than other levels and status. About 86 % of the studied women had vaginal discharge , while 81% of them had vulvular itching . Dysuria was Other less frequent symptom. These results indicated the low prevalence of Trichomonas vaginalis among Iraqi women at Wasit province

This study was concerned the describe of an current PCR method for specific detection of Tichomonas vaginalis DNA into a rapid nested PCR assay, and to estimate the recent prevalence of T. vaginalis according to studying different factors that effect on vaginal trichomoniasis in women complaining of vaginal discharge. Vaginal swab samples were collected from 100 patients lived at Wasit province, during the period from January, 2022 to July, 2022. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 100 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid nested PCR. All samples were examined by the direct microscopic examination (wet mount). The vaginal swabs examined by wet mount and nested PCR to detect the presence of T. vaginalis. Of the 100 women, 29 were positive by nested PCR giving the prevalence rate of (29%) with and the direct microscopic examination (wet mount) showed 4/100 (4 %) positive case. Their ages were different. Women aged (20-30)years had significantly higher prevalence of trichomoniasis (14%) than other age groups.