Objectives The present study aims to inspect the connection between patients with SLE and RF, CRP, Brucellosis and compression between immunofluorescence technique and latex agglutination method to detect ANA. Methods A total of 90 serum sample collected from patients with symptoms and signs of SLE those send to the immunity lab in Al-Hussein hospital. The immunofloriscent ANA test, SLE latex agglutination test, RF, CRP and Rose Bengal tests were done to all these samples. Results The results show that there was significant difference between males (16%) and females (84%) patients with sign and symptom of SLE, while there was no significant difference in CRP, RF, in patients with SLE positive (50%,17%) and SLE negative patients (48.5%,17%), continuously Rose Bengal test is higher in SLE negative patients than in SLE positive patients (15.2%, 8.3%). Finally, there is significant difference in diagnosis of SLE disease between IF technique and latex agglutination test (41.6%, 100%). Conclusions Latex agglutination test for ANA detection is reliable, specific but less sensitive test when compared to SLE IF test. There are significance differences in Rose Bengal test in SLE negative and in SLE positive patient's, while there are non-significance difference in CRP, RF in SLE positive samples and in SLE negative samples.