In this study, three different self emulsifying drug delivery systems of rifampicin (SEDD-R) were made using oleic acid and different surfactants such as Tween 80, Chremophor RH 40 and Chremophor EL designated as RN-TW, RN-CRH and RN-CEL. These self-emulsifying systems were converted to rifampicin nanoemulsion by adding water under sonication. The resulting particle sizes were found to be 192. Antibacterial effect of the preparations was evaluated against S. epidermidis and S. aureus isolated from acne and showed effective results.