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This paper is a comparative study on the preparation techniques used to make the support layer of polyamide‐thin‐film composite forward osmosis (TFC‐FO) membranes. The role played by the support layer preparation technique in membrane performance is thoroughly investigated in this study. Electrospinning is shown to produce membranes of lower structural parameter compared to those obtained by conventional phase inversion techniques. The electrospun polyamide selective layer can also be tailored with the required properties. This makes electrospinning a promising process to design efficient FO membrane substrates. It is shown in this work that the FO water flux is more dependent on the internal structure of the support layer than the preparation materials. The main challenge remaining for substrates to operate in FO is to achieve simultaneously a low structural parameter, a high surface porosity, and the required mechanical properties. As most of today's approaches are not suitable, further materials development is essential in future investigations on TFC‐FO membranes.

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