Background To evaluate the use of allogenic freeze-dry PRP for the full-thickness wound healing process in experimental animals (rabbits). Material and methods This study is an experimental research using the posttest-only design, a simple randomized design. Thirty rabbits were randomly divided into six groups (three control groups and three treatment groups), and each group consisted of five subjects. Skin-thick cuts were made on the back of each rabbit. Wounds in the control group were treated using a paraffin gauze, and allogenic freeze-dried PRP was given to the treatment group; observations were made on the 3rd, 9th, and 14th days. The t -test on two random samples and Mann–Whitney were used to process data. The results were significant at p < 0.05. Results There were significant differences in the average number of fibroblasts the control groups and the treatment groups on day 3 (p = 0.009), day 9 (p = 0.023), and day 14 (p < 0.001). There were also major differences in the average amount of neovascularization between the control group and the treatment group on day 3 (p = 0.034), day 9 (p < 0.001), and day 14 (p < 0.001). Conclusions The application of allogenic freeze-dried PRP was confirmed to be able to increase the number of fibroblasts and neovascularization in the full-thickness wound healing process in rabbits.

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BACKGROUND: We investigated the epidemiology and the knowledge of first aid regarding to burns in the rural area of Kulon Progo, located in Indonesia. Although 44% of Indonesia’s population resides in rural areas, data of burn in Indonesia are very limited to national referral centers located in Indonesia’s largest cities. We also surveyed the patients and families of these burn patients to try to understand their knowledge on the first aid of burns. AIM: We wanted to explore and discover what myths and misperceptions existed in rural communities. MATERIALS AND METHODS: A retrospective study analyzing the medical records of patients with burns admitted to two of Kulon Progo’s largest referral hospitals between January 2018 and December 2019. A survey was distributed to the patients and their families regarding their knowledge on the first aid of burns. We wanted to analyze if certain groups of the population were more at risk for specific types of burn etiologies, the correlation between burn etiology and length of stay, the correlation between total body surface area (TBSA) of burns and length of stay in the hospital, and the correlation between education level of survey participants’ and their knowledge of first aid of burns. RESULTS: A total of 115 patients were reviewed in this study. The highest proportion of total burn injuries occurred in the age group of 1–14 years old (38%). The major etiology of all burn cases were caused by scald (54%). Housewives and children were at a higher risk for scalds and laborers were at a higher risk for electrical and chemical burns (p = 0.001). There was no significant correlation between etiology and length of stay (p = 0.29). There was a statistically significant correlation between the TBSA and the length of hospital stay (p = 0.0001). The majority of survey respondents (66%) had poor knowledge on the first aid of burns. There was no significant correlation between education level and knowledge of first aid of burns (p = 0.07547). CONCLUSIONS: Children, laborers, and housewives have a significant risk of suffering from a burn injury. A majority of people still have poor knowledge on the first aid of burns. There was no correlation between education levels and knowledge of first aid burns.

Background: The incidence of burns in Indonesia progressively increases with the increase in its population and industries. From January to September 2000, 158 patients were treated in the burn unit of Dr Soetomo Hospital with a mortality rate reaching 5,8%. Burns have a direct effect in causing both local and systemic changes in the body, not occurring in other injuries. In severe burns, a hypermetabolic state can occur, which increases cardiac workload and causes muscle atrophy and other morbidities. The purpose of this study is to examine the effect of propranolol on the hypermetabolic state in severely burned patients by measuring various clinical & laboratory parameters.Methods : This is an experimental study using pre and post test control group design with the objective of assessing the treatment outcome with oral propranolol given in 15 consecutive days for burn patients involving 25%- 60% TBSA. Measurements were taken three times, on day 0, 7 and 14.Results : Obtained 16 samples divided into 2 groups. In the treatment group, there was a significant decrease in CRP levels on days 0, 7 and 14 (p <0.05). The Mid Arm Circumference variable did not obtain a significant decreasing on days 0, 7 and 14. The albumin level studied showed a significant decreasing on day 0 & 7 days with a value of p = 0.045. From the comparison between the two groups, there were significant differences in CRP levels on days 0 & 14, with the mean value of the treatment group -5.12 + 2.88 and the mean value of the control group 2.86 + 7.37, and the value of p = 0.019.Conclusions: This study successfully proved that the administration of propranolol can overcome the effects of hypermetabolism which is characterized by decreasing in CRP levels.

Latar Belakang: Luka bakar merupakan salah satu masalah global di Indonesia. Infeksi pada pasien luka bakar merupakan salah satu faktor yang mempengaruhi lama masa perawatan berkaitan dengan tingkat patogenisitas bakteri penyebab infeksi. Tujuan penelitian ini adalah untuk mengetahui hubungan antara lama masa perawatan dengan jenis kuman penyebab infeksi pada pasien luka bakar yang dirawat di RSUD Dr. Soetomo Surabaya periode Januari-Desember 2019. Metode: Penelitian ini merupakan studi deskriptif analitik dengan desain kohort retrospektif. Data mengenai karakteristik pasien, derajat keparahan luka bakar, penyakit penyerta, jenis bakteri yang menginfeksi pasien luka bakar, serta lama masa perawatan didapatkan dari rekam medis pasien. Data mengenai jenis bakteri yang menginfeksi pasien luka bakar dan lama perawatan pasien luka bakar ditentukan korelasinya dengan menggunakan uji statistik Rank Spearman-Rho. Hasil: Selama kurun waktu penelitian diperoleh sebanyak 42 data pasien luka bakar yang mengalami infeksi dan didominasi pasien berjenis kelamin laki-laki (61,9%). Persentase rentang usia terbanyak adalah 26-35 tahun yakni 23,8%. Persentase derajat keparahan luka bakar terbanyak adalah derajat II yang didominasi derajat IIB yakni 38,1%. Gangguan metabolik merupakan kondisi penyerta tersering yang dialami pasien luka bakar yakni sebanyak 50%. Rerata lama perawatan pasien luka bakar yang mengalami infeksi adalah 28,21+10,17 hari dengan waktu paling lama adalah 53 hari. Jenis kuman yang menginfeksi pasien luka bakar pada penelitian ini didominasi oleh Acinetobacter baumanii (31%), Staphylococcus haemolyticus (23,8%), dan Pseudomonas aeruginosa (16,7%). Uji korelasi antara lama masa perawatan pada pasien luka bakar dan jenis bakteri yang menginfeksi pasien luka bakar menunjukkan hasil yang signifikan (p = 0,012). (nomer kelaikan etik : 0248/LOE/301.4.2/XII/2020) Kesimpulan: Terdapat korelasi yang signifikan antara lama masa perawatan dengan jenis bakteri yang menginfeksi pasien luka bakar.