Hypercholesterolemia is an important risk factors of atherosclerosis. Bitter leaf is believed to have potency in decreasing plasma lipid. The aim of this study is to study the effect of bitter leaf ethanol extract in decreasing total cholesterol serum levels on hypercholesterolemic male Wistar rats. This is a true experimental laboratory study with complete randomized design. Antidyslipidemia effect was tested with HFF induction and Propiltiourasil 0,01% towards 30 rats which were divided into 6 groups: groups I as normal control (standard diet), groups II, III, and IV were given bitter leaf ethanol extract of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW dose, group V as positive control group with Simvastatin, and group VI as negative with aquades. HFF  were given for 14 days then the study was continued for 14 days. The percentage data of the declining total cholesterol levels before and after treatments is analyzed with ANAVA, continued with LSD test. The result between group II, III, and IV with group VI shows a significant difference p<0,01. We concluded bitter leaf ethanol extract can decrease the level of total cholesterol serum of hypercholesterolmic Wistar rats.