This research was conducted in order to determine a potential use of waste sludge of animal origin as soil amendment in the relation to the content of macroelements N, P and S and to determine effect of sludge application on the quantity and quality of lettuce (Lactuca sativa L.). The experiment was set up in 3 treatments with 3 replications. Treatments included control and two different amounts of animal waste sludge which was used as soil amendment (fertilization) for lettuce. The study investigated crystal green salad sort Posavka because of good accumulation ability to N (nitrophilic plant). Results showed that content of total N in waste animal sludge was 7.48%, total P was 1% and content of total S was 1.55%. Statistical analysis showed no significant difference in yield between the treatments. The content of NO3 -in lettuce was not statistically significant between the treatments and the values were less than those prescribed by the legislation, which lead us to conclusion of safety use of sludge regarding the nitrates. According to the results of test parameters it can be cocluded that the animal waste sludge is well provided with macroelements (N, P and S) and suitable for use in agriculture as an amendment for improving soil quality. Certainly, for crops that are not used for human consumption, with additional obligated control of other chemical and microbiological parameter before the use.