ABSTRACT. The influence of copper on the ciliate Euplotes sp. and associated bacteria isolated from sediment samples of Guanabara Bay were investigated in bioassays. This region is highly affected by heavy metals such as copper, from solid waste constantly dumped in the bay and other sources such as industrial effluents, antifouling paints, atmospheric deposition and urban drainage, and even today there are few data on the metal toxicity to the ecosystem of the Bay of Guanabara. Bioassays were conducted to estimate the LC 50 -24 h of copper, in order to determine the concentration of metal bearing 50% of the population mortality. The results indicated that the concentrations of 0.05 and 0.009 mg L -1 presented no toxicity to Euplotes sp. The associated bacteria are tolerant to copper concentrations used in bioassays, and suggest that they could be used as a potential agent in the bioremediation of areas affected by copper. Keywords: Euplotes sp., bacteria, copper, heavy metals, Guanabara Bay, southeast Brazil.Influencia del cobre sobre Euplotes sp. y la población de bacterias asociada RESUMEN. La influencia del cobre sobre el ciliado Euplotes sp. y las bacterias asociadas aisladas de muestras de sedimentos de la bahía de Guanabara fueron investigados en bioensayos. Esta región está altamente afectada por metales pesados, como el cobre, a partir de residuos sólidos constantemente vertidos en la bahía y de otras fuentes, como los efluentes industriales, pinturas antiincrustantes, deposición atmosférica y drenaje urbano, y hasta hoy hay pocos datos sobre la toxicidad del metal para el ecosistema de la bahía de Guanabara. Se efectuaron bioensayos para estimar la CL 50 -24 h de cobre para determinar la concentración de metales que lleva al 50% de la mortalidad de la población. Los resultados indicaron que las concentraciones de 0,05 y 0,009 mg L -1 , no representan toxicidad para Euplotes sp. Las bacterias asociadas son tolerantes a las concentraciones de cobre utilizadas en los bioensayos, lo que sugiere que podrían ser utilizadas como un agente potencial en la biorremediación de las zonas afectadas por el cobre. Palabras clave: Euplotes sp., bacterias, cobre, metales pesados, bahía de Guanabara, sureste de Brasil.
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