No abstract

T Th he e l lu un ng g i in n c cl lo os se ev vi ie ew w: : a a c co or rr ro os si io on n c ca as st ti in ng g s st tu ud dy y o on n t th he e v va as sc cu u--l la ar r s sy ys st te em m o of f h hu um ma an n f fo oe et ta al l t tr ra ac ch he ea a ABSTRACT: The aim of this study was to examine the tracheal vasculature in 5 month human foetuses, and to determine whether it differs from that previously described by other authors for adult human trachea. The vascular bed was visualized using the technique of corrosion casting and examined by scanning electron microscopy.The arrangement of larger vessels: longitudinal tracheo-oesophageal arteries and veins, as well as their segmental branches running circumferentially in the intercartilaginous spaces, was similar to that observed in the trachea of adults. However, no blood sinuses reported to occur in the submucous venular plexus of the human trachea could be found in the foetuses. It is postulated that the possible functions of such sinuses acting as the capacitance system are related to the functional respiratory tract; hence, this vascular specialization is not yet developed in the foetus.

The underdevelopment of some vascular areas is postulated to reflect the functional immaturity of the respiratory system in the fetus.

Galanin exerts tonic inhibition of nociceptive input to the central nervous system. Recently, this peptide was demonstrated in several neuronal and non-neuronal structures in bones and joints. In this study, the time of appearance and topographic localization of galanin-containing nerve fibres in bone were studied in rats from gestational day 16 (GD16) to postnatal day 21 (PD21). The tibia was chosen as a model of developing long bone and indirect immunofluorescence combined with confocal laser scanning microscopy was used to identify galanin-immunoreactive (GAL-IR) nerve fibres. The earliest, sparse GAL-IR fibres were observed on GD21 in the perichondrium of both epiphyses and in the periosteum of the diaphysis. From PD1 onwards, GAL-IR fibres were also seen in the bone marrow cavity and in the region of the inter-condylar eminence of the knee joint. Intramedullary GAL-IR fibres in proximal and distal metaphyses appeared around PD1. Some of them accompanied blood vessels, although free fibres were also seen. GAL-IR fibres located in the cartilage canals of both epiphyses were observed from PD7, in the secondary ossification centres from PD10 and in the bone marrow of both epiphyses from PD14. The time course and localization of galanin-containing nerve fibres resemble the development of substance P- and CGRP-expressing nerve fibres, thus suggesting their sensory origin.

Surfaces of aural polyps collected from 30 patients were examined by scanning electron microscopy. In the polyps not associated with cholesteatoma, the epithelial lining showed individually variable metaplasia towards cuboidal 'cobblestone'-type and squamous epithelium covered with microvilli of various shapes and sizes. Squamous epithelium was present on the surface of all polyps with underlying cholesteatoma, with superficial cells possessing elongated microvilli, microplicae of different sizes, grooves and pits. Such surface structures reflect different stages of the keratinization process that seems to be characteristic for the epithelial lining of polyps with underlying cholesteatoma. Incomplete epithelium accompanied by granulation tissue was found in several polyps; in two cholesteatoma-associated polyps plate-like cholesterol crystals were observed.