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185 186 J . A. NISSIMwhich had daily injections of 11.25 mg. Fe/kg. subcutaneously as 0.3 per cent. Fe solution, the total iron given being 0.17, 0.28, 0.5 and 1.0 g. Fe/kg. ; two guinea-pigs received 0.17 and 0.2 g. Fe/kg. intraperitoneally.The histological technique was designed to preserve the plasma in the heart and large vessels, so that the state and concentration of circulating iron could be studied. The best results were obtained with Cappell's techniqiie of immersing the animals whole in 10 per cent. formol-saline for 24 hours and injecting the pleural and peritoneal cavities with the fixative. The iron was stained by treatment with 4 per cent. potassium ferrocyanide and the Prussian blue showed best when sections were counterstained with carmrtlum. Eosin was found t o mask diffuse iron staining in tissues.